Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Exploring the Continuing Education Experiences and Needs of Hearing Health Care Professionals: A Survey-Based Study.

American journal of audiology·2026
Same author

Epithelial MLCK deficiency alleviates alcohol-associated liver disease via dendritic-Th17 cell axis.

JHEP reports : innovation in hepatology·2026
Same author

Spatial integration of protein and chromosomal states reveals early copy number changes and genotype-associated immune neighborhoods in serous ovarian cancer evolution.

Cancer discovery·2026
Same author

The end of 'leaky gut': pathway-specific mechanistic approaches to barrier restoration.

Current opinion in gastroenterology·2026
Same author

Macrophage-derived cathepsin B disrupts intestinal tight junctions through occludin degradation and promotes alcohol-associated liver disease.

Journal of hepatology·2026
Same author

Targeting MLCK1 uncouples immune checkpoint inhibitor-induced colitis from antitumour immunity.

Gut·2026
Same journal

A Novel Laboratorial Approach to Evaluate Bacterial Microleakage of Endodontic Sealers.

Current protocols·2026
Same journal

TRIAGE Toolkit: Streamlined Discovery of Regulatory Genes and Elements.

Current protocols·2026
Same journal

High-throughput Profiling of Pseudouridines in Microbiome-derived Bacterial RNA.

Current protocols·2026
Same journal

Recombinant Protein Expression in Rhodococcus species.

Current protocols·2026
Same journal

Streamlined In Vitro Transcription for Generating Self-Amplifying RNA With Modified Nucleotides.

Current protocols·2026
Same journal

CODEC Library Preparation From Genomic DNA.

Current protocols·2026
See all related articles

Related Experiment Video

Updated: Nov 10, 2025

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples
08:18

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples

Published on: April 7, 2023

1.9K

A Simple Method for Creating a High-Content Microscope for Imaging Multiplexed Tissue Microarrays.

Shabnam Abtahi1, Neal R Gliksman2, John F Heneghan1

  • 1Department of Pathology, Laboratory of Mucosal Barrier Pathobiology, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts.

Current Protocols
|April 6, 2021
PubMed
Summary
This summary is machine-generated.

This study presents a cost-effective method for slide scanning using any standard microscope, enabling digital archiving and virtual microscopy. The protocol allows automated acquisition of multiple regions of interest (ROIs) for high-throughput imaging.

Keywords:
automated image acquisitiondigital pathologyfluorescence microscopyslide scanningtissue microarray

More Related Videos

Author Spotlight: Introducing the Tile/SED/Array Interface for Rapid Field of View Positioning in Tissue Imaging
06:15

Author Spotlight: Introducing the Tile/SED/Array Interface for Rapid Field of View Positioning in Tissue Imaging

Published on: September 15, 2023

680
A Rapid Method for Multispectral Fluorescence Imaging of Frozen Tissue Sections
07:50

A Rapid Method for Multispectral Fluorescence Imaging of Frozen Tissue Sections

Published on: March 30, 2020

9.7K

Related Experiment Videos

Last Updated: Nov 10, 2025

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples
08:18

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples

Published on: April 7, 2023

1.9K
Author Spotlight: Introducing the Tile/SED/Array Interface for Rapid Field of View Positioning in Tissue Imaging
06:15

Author Spotlight: Introducing the Tile/SED/Array Interface for Rapid Field of View Positioning in Tissue Imaging

Published on: September 15, 2023

680
A Rapid Method for Multispectral Fluorescence Imaging of Frozen Tissue Sections
07:50

A Rapid Method for Multispectral Fluorescence Imaging of Frozen Tissue Sections

Published on: March 30, 2020

9.7K

Area of Science:

  • Microscopy and Imaging
  • Digital Pathology
  • Biotechnology

Background:

  • High-throughput, high-content imaging and multiplex slide scanning are valuable but often limited by dedicated device cost and inflexibility.
  • Existing methods require specialized, expensive equipment, hindering broader adoption in research settings.

Purpose of the Study:

  • To describe a simple, adaptable method for slide scanning using any standard microscope.
  • To enable digital slide archiving, virtual microscopy, and standardized analysis without specialized hardware.

Main Methods:

  • Utilizes a microscope with a motorized filter turret and XYZ stage, controlled by microscope software capable of routine programming.
  • Defines regions of interest (ROIs) and imaging parameters through a series of automated command routines (journals).
  • Automated acquisition of tiled images, followed by stitching and assembly using image-processing applications.

Main Results:

  • The method allows any microscope with motorized components to perform slide scanning.
  • Automated acquisition of fifty 2.5-mm ROIs in four channels is achieved in under 3 hours.
  • Images can be readily stitched and displayed using open-source or commercial software for virtual microscopy.

Conclusions:

  • This protocol offers a flexible and economical alternative to dedicated slide scanners.
  • It facilitates digital slide archiving, team-based review, and standardized analysis in various research fields.
  • The method supports multiplex immunostaining and background subtraction for advanced imaging applications.