Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Riboswitches01:56

Riboswitches

9.0K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
9.0K
Alternative RNA Splicing02:18

Alternative RNA Splicing

23.6K
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
23.6K
Alternative RNA Splicing02:18

Alternative RNA Splicing

4.4K
4.4K
Pre-mRNA Processing: RNA Splicing01:36

Pre-mRNA Processing: RNA Splicing

6.0K
6.0K
RNA Splicing01:32

RNA Splicing

58.5K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
58.5K
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

7.6K
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
7.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Distinct signaling mechanisms of constitutively active phytochromes A and B in regulating Arabidopsis seedling development.

Plant physiology and biochemistry : PPB·2025
Same author

LHP1 and INO80 cooperate with ethylene signaling for warm ambient temperature response by activating specific bivalent genes.

Cell reports·2024
Same author

A simple method to visualize pre-mRNA splicing with the naked eye using a genetically encoded visual splicing reporter.

Plant physiology·2024
Same author

PHOSPHATASE 2A dephosphorylates PHYTOCHROME-INTERACTING FACTOR3 to modulate photomorphogenesis in Arabidopsis.

The Plant cell·2024
Same author

Shining light on plant growth: recent insights into phytochrome-interacting factors.

Journal of experimental botany·2024
Same author

ELONGATED HYPOCOTYL 5 interacts with HISTONE DEACETYLASE 9 to suppress glucosinolate biosynthesis in Arabidopsis.

Plant physiology·2024

Related Experiment Video

Updated: Nov 10, 2025

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
08:53

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

3.0K

Light-regulated pre-mRNA splicing in plants.

Praveen Kumar Kathare1, Enamul Huq1

  • 1Department of Molecular Biosciences, The Institute for Cellular and Molecular Biology, The University of Texas at Austin, Austin, TX 78712, USA.

Current Opinion in Plant Biology
|April 6, 2021
PubMed
Summary

Phytochromes (phys) regulate plant development by controlling gene expression. These photoreceptors modulate pre-mRNA splicing in response to light, influencing transcript diversity and promoting photomorphogenesis.

Keywords:
ArabidopsisPhotomorphogenesisPhytochrome signalingPre-mRNA splicingSplicing factor

More Related Videos

mRNA Interactome Capture from Plant Protoplasts
12:29

mRNA Interactome Capture from Plant Protoplasts

Published on: July 28, 2017

9.3K
Using the E1A Minigene Tool to Study mRNA Splicing Changes
10:25

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

5.2K

Related Experiment Videos

Last Updated: Nov 10, 2025

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
08:53

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

3.0K
mRNA Interactome Capture from Plant Protoplasts
12:29

mRNA Interactome Capture from Plant Protoplasts

Published on: July 28, 2017

9.3K
Using the E1A Minigene Tool to Study mRNA Splicing Changes
10:25

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

5.2K

Area of Science:

  • Plant biology
  • Molecular genetics
  • Photomorphogenesis

Background:

  • Phytochromes (phys) are photoreceptors that regulate plant growth and development in response to light signals.
  • Light-mediated physiological responses are controlled by phytochromes through gene expression regulation at transcriptional and post-transcriptional levels.

Purpose of the Study:

  • To summarize the current understanding of how phytochromes regulate pre-mRNA splicing in response to light.
  • To elucidate the contribution of phytochrome-modulated splicing to gene expression and photomorphogenesis.

Main Methods:

  • Review of recent large-scale RNA-sequencing studies.
  • Analysis of identified phytochrome-interacting splicing factors/regulators.
  • Forward genetics and protein-protein interaction studies.

Main Results:

  • Phytochromes alter global transcript diversity by modulating pre-mRNA splicing in response to light.
  • Several phytochrome-interacting splicing factors have been identified across different species.
  • These factors play a role in light-regulated pre-mRNA splicing.

Conclusions:

  • Phytochromes are crucial regulators of light-mediated pre-mRNA splicing.
  • Splicing modulation by phytochromes contributes significantly to gene expression regulation.
  • This mechanism is essential for promoting photomorphogenesis in plants.