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Comprehensive evaluation of Licorice extract by five-dimensional quantitative profiling.

Lili Lan1, Wanyang Sun2, Qian Chang1

  • 1School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, Liaoning 110016, China.

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|April 6, 2021
PubMed
Summary

This study introduces a five-dimensional profiling method (FDPM) to assess licorice extract (LE) quality by analyzing five active components and its HPLC fingerprint. FDPM effectively categorizes LE batches, ensuring consistent quality control in food and medicine.

Keywords:
Fingerprint system calibrationFive-dimensional profiling methodHPLCLicorice extractQuality control

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Area of Science:

  • Phytochemistry
  • Analytical Chemistry
  • Quality Control

Background:

  • Licorice extract (LE) is utilized in food and medicine, but single-component analysis is insufficient for quality assessment.
  • Developing a comprehensive method for LE quality evaluation is crucial.

Purpose of the Study:

  • To establish a robust method for evaluating the overall quality of licorice extract.
  • To address the limitations of single-component detection in quality control.

Main Methods:

  • Developed a dual-standard system calibration to standardize chromatographic systems.
  • Established a five-dimensional profiling method (FDPM) analyzing five active components and HPLC fingerprint.
  • Utilized cluster analysis and Orthogonal projections on the latent structure - discrimination analysis (OPLS-DA) for quality assessment.

Main Results:

  • FDPM successfully evaluated 52 batches of LE, dividing them into four distinct categories.
  • The method demonstrated that quality and quantity of LE can be effectively reflected by FDPM.
  • FDPM identified potential information for monitoring and calibrating chromatographic systems.

Conclusions:

  • The five-dimensional profiling method (FDPM) provides a comprehensive approach to licorice extract quality control.
  • FDPM offers strategies for monitoring and calibrating chromatographic fingerprint systems.
  • This method enhances the reliability of LE quality assessment in various industries.