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Kinetic properties from bass liver 6-phosphogluconolactonase
M M Medina-Puerta1, M Gallego-Iniesta, A Garrido-Pertierra
1Departamento de Bioquímica de la Facultad de Veterinaria de la Universidad Complutense, Spain.
Biochemistry International
|March 1, 1988
Abstract:
By specific enzymic synthesis of the substrate 6-phosphogluconolactone "in situ", the Km for bass liver 6-phosphogluconolactonase was found to be 90 microM. This value is compatible with the kinetic parameters of both glucose 6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase from bass liver, and hence with the flux through the oxidative phase of the pentose phosphate pathway.