Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Highly effective complementary addressed laser modification (cleavage) of oligodeoxynucleotides].

L Z Benimetskaia, N V Bulychev, A L Kozionov

    Bioorganicheskaia Khimiia
    |January 1, 1988
    PubMed
    Summary

    Nonlinear photomodification of DNA targets was achieved using fluorescently labeled oligonucleotides. This method enables targeted DNA cleavage and modification with high efficiency, offering a novel tool for molecular biology applications.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    The features of Tat protein of human immunodeficiency virus type 1 (Retroviridae: <i>Lentivirus: Lentivirus humimdef1</i>) non-A6 variants, characteristic for the Russian Federation.

    Voprosy virusologii·2025
    Same author

    Variability of non-structural proteins of HIV-1 sub-subtype A6 (Retroviridae: Orthoretrovirinae: Lentivirus: Human immunodeficiency virus-1, sub-subtype A6) variants circulating in different regions of the Russian Federation.

    Voprosy virusologii·2024
    Same author

    Efficacy of first-line ART regimens based on tenofovir in HIV-infected patients with pre-existing A62V mutation in reverse transcriptase.

    Voprosy virusologii·2024
    Same author

    Cognitive flexibility is associated with sickness resilience.

    Frontiers in psychology·2024
    Same author

    [Genetic diversity of capsid protein (p24) in human immunodeficiency virus type-1 (HIV-1) variants circulating in the Russian Federation].

    Voprosy virusologii·2023
    Same author

    [Comparative analysis of genetic variants of the HIV-1 circulating in the Irkutsk region in 1999 and 2012].

    Voprosy virusologii·2022

    Area of Science:

    • Oligonucleotide chemistry
    • Photochemistry
    • Molecular biology

    Context:

    • Oligodeoxynucleotides are crucial in molecular biology and therapeutics.
    • Precise modification of DNA is essential for various applications.
    • Nonlinear optical processes offer unique photochemical control.

    Purpose:

    • To investigate complementary addressed nonlinear photomodification of an oligodeoxynucleotide target.
    • To utilize fluorescently labeled oligonucleotides as "addressed chromophores" for photomodification.
    • To characterize the efficiency and mechanism of photomodification, including cleavage and adduct formation.

    Summary:

    • Complementary addressed nonlinear photomodification of a DNA target was performed using ethidium-dye-labeled oligonucleotides and nitrogen laser irradiation.

    Related Experiment Videos

  • Photomodification resulted in target cleavage (up to 10%), covalent adduct formation (20-70%), and "hidden" damages (7-27%), with total specific modification yields of 50-80%.
  • Piperidine treatment enhanced addressed cleavage to 40%, and the observed modifications were independent of radical scavengers, supporting a two-photon excitation energy transfer mechanism.

    • Impact:

    • Demonstrates a novel method for site-specific DNA modification using nonlinear photochemistry.
    • Offers potential for developing new tools in molecular biology, diagnostics, and therapeutics.
    • Highlights the utility of optically nonlinear processes for precise molecular manipulation.