Related Experiment Video
Updated: Nov 9, 2025
Studying Orthodontic Tooth Movement in Mice
Published on: August 2, 2024
Comparison of tooth movement and biological response resulting from different force magnitudes combined with
Cheng-Yi Huang1, Hai-Ping Lu2, Yu-Feng Yu3
1Zhejiang University School of Medicine, Sir Run Run Shaw Hospital, Department of Dentistry, Center of Orthodontics, Hangzhou, China.
Objective:
To compare tooth movement rate and histological responses with three different force magnitude designs under osteoperforation in rabbit models.
Methodology:
48 rabbits were divided into three groups: Group A, Group B, and Group C, with traction force of 50 g, 100 g, 150 g, respectively. Osteoperforation was performed at the mesial of the right mandibular first premolar, the left side was not affected. One mini-screw was inserted into bones between two central incisors. Coil springs were fixed to the first premolars and the mini-screw. Tooth movement distance was calculated, and immunohistochemical staining of PCNA, OCN, VEGF, and TGF-β1 was analyzed.
Results:
The tooth movement distance on the surgical side was larger than the control side in all groups (P<0.01). No significant intergroup difference was observed for the surgical side in tooth movement distance among the three groups (P>0.05). For the control side, tooth movement distance in Group A was significantly smaller than Groups B and C (P<0.001); no significant difference in tooth movement distance between Group B and Group C was observed (P>0.05). On the tension area of the moving premolar, labeling of PCNA, OCN, VEGF and TGF-β1 were confirmed in alveolar bone and periodontal ligament in all groups. PCNA, OCN, VEGF and TGF-β1 on the surgical side was larger than the control side in all groups (P<0.001).
Conclusion:
Osteoperforation could accelerate orthodontic tooth movement rate in rabbits. Fast osteoperforation-assisted tooth movement in rabbits was achieve with light 50 g traction.
