Related Experiment Video
Updated: Nov 9, 2025

High-Speed Ultraviolet Photoacoustic Microscopy for Histological Imaging with Virtual-Staining assisted by Deep Learning
Published on: April 28, 2022
A through-focus scanning optical microscopy dimensional measurement method based on deep-learning classification
Haitao Nie1, Renju Peng1, Jiajun Ren1
1School of Instrumentation and Opto-Electronic Engineering, Beihang University, Beijing, China.
Abstract:
Through-focus scanning optical microscopy (TSOM) is an economical, non-contact and nondestructive method for rapid measurement of three-dimensional nanostructures. There are two methods using TSOM image to measure the dimensions of one sample, including the library-matching method and the machine-learning regression method. The first has the defects of small measurement range and strict environmental requirements; the other has the disadvantages of feature extraction method greatly influenced by human subjectivity and low measurement accuracy. To solve the problems above, a TSOM dimensional measurement method based on deep-learning classification model is proposed. TSOM images are used to train the ResNet50 and DenseNet121 classification model respectively in this paper, and the test images are used to test the model, the classification result of which is taken as the measurement value. The test results showed that with the number of training linewidths increasing, the mean square error (MSE) of the test images is 21.05 nm² for DenseNet121 model and 31.84 nm² for ResNet50 model, both far lower than machine-learning regression method, and the measurement accuracy is significantly improved. The feasibility of using deep-learning classification model, instead of machine-learning regression model, for dimensional measurement is verified, providing a theoretical basis for further improvement on the accuracy of dimensional measurement.
Related Concept Videos
Confocal Fluorescence Microscopy
Three-Dimensional Microscopy in Microbiology
Overview of Microscopy Techniques
Atomic Force Microscopy
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
Super-resolution Fluorescence Microscopy

