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Updated: Nov 9, 2025

Author Spotlight: Revolutionizing Microfluidics Through Microchannel Fabrication on Nanopaper
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Aptamer-Integrated Multianalyte-Detecting Paper Electrochemical Device.

Yingzhu Liu1, Obtin Alkhamis1, Xintong Liu1

  • 1Department of Chemistry and Biochemistry, Florida International University, 11200 SW Eighth Street, Miami, Florida 33199, United States.

ACS Applied Materials & Interfaces
|April 7, 2021
PubMed
Summary
This summary is machine-generated.

Researchers developed multiplex paper electrochemical devices (mPEDs) for on-site detection of multiple small molecules. These user-friendly biosensors offer rapid, specific, and sensitive analysis in complex samples using aptamer modifications.

Keywords:
ambient vacuum filtrationaptamermultianalyte detectionpaper electrochemical devicesensingsmall molecules

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Area of Science:

  • Biosensing and Analytical Chemistry
  • Nanomaterials and Nanotechnology
  • Biotechnology and Bioengineering

Background:

  • On-site detection of multiple small-molecule analytes in complex samples is crucial for various biosensing applications.
  • Paper electrochemical devices (PEDs) are promising for point-of-care diagnostics due to their low cost, portability, and ease of use.
  • Existing methods often lack the multiplexing capability or robustness required for complex biological matrices.

Purpose of the Study:

  • To develop a simple, inexpensive, and reproducible fabrication method for aptamer-modified multiplex paper electrochemical devices (mPEDs).
  • To demonstrate the capability of mPEDs for robust and specific on-site detection of multiple small molecules in complex samples.
  • To enable rapid, sensitive, and accurate multi-analyte detection in a single sample using a drop of biological fluid.

Main Methods:

  • Fabrication of mPEDs using an ambient vacuum filtration technique with carbon and metal nanomaterials.
  • Development of a sensing architecture with a silver pseudo-reference electrode, gold counter electrode, and three gold working electrodes.
  • Modification of individual working electrodes with different aptamers for specific small-molecule analyte recognition.

Main Results:

  • Successfully fabricated user-friendly and highly reproducible aptamer-modified mPEDs.
  • Demonstrated sensitive and accurate detection of multiple small-molecule analytes simultaneously in complex samples within seconds.
  • Showcased the utility of an integrated PDMS chamber for detection using only microliter volumes of biological samples.

Conclusions:

  • The developed mPEDs offer a facile and effective platform for on-site, multi-analyte detection of small molecules.
  • The fabrication technique is generalizable and adaptable for a wide range of aptamer-target combinations.
  • This approach paves the way for future advancements in point-of-care diagnostics and personalized medicine through rapid on-site analysis.