Topological distribution of choline phospholipid fatty acids in trout intestinal brush-border membrane

The transbilayer distribution of choline phospholipids in trout intestinal brush-border membrane has been investigated using phospholipase C (from Clostridium welchii). In the middle intestine, 84% of phosphatidylcholine (PC) and 60% of sphingomyelin (SP) are located in the outer membrane leaflet. In the posterior intestine, 89% of PC and 52% of SP are located in the outer membrane leaflet. The externally located PC molecular species are (n - 3) fatty acid-rich in both parts of the intestine. While the sphingomyelin molecular species containing 24:1(n - 9) are exclusively located in the outer leaflet in the middle intestine, those containing 14:0 are more abundant in the same leaflet but in the posterior intestine. This strongly asymmetric distribution of both choline phospholipids may have numerous consequences on the brush-border membrane characteristics.