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Protein Conjugation via SpyStapler-Mediated SpyTag/BDTag Coupling

Xiao-Di Da1, Xia-Ling Wu1, Yajie Liu1

  • 1Beijing National Laboratory for Molecular Sciences, Key Laboratory of Polymer Chemistry & Physics of Ministry of Education, Center for Soft Matter Science and Engineering, College of Chemistry and Molecular Engineering, Peking University, Beijing, P. R. China.

Current Protocols
|April 7, 2021
PubMed
Summary

SpyStapler technology enables efficient protein bioconjugation using short peptide tags for isopeptide or mechanical bond formation. This method simplifies creating large protein conjugates, potentially enhancing therapeutic properties.

Keywords:
SpyCatcherSpyStaplerSpyTagbioconjugationprotein catenane

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