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CRISPR-Cas9-Mediated Genome Editing in the Filamentous Ascomycete Huntiella omanensis
Published on: June 9, 2020
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CRISPR/Cas Genome Editing in Filamentous Fungi
Aleksandra M Rozhkova1, Valeriy Yu Kislitsin2
1Bach Institute of Biochemistry, Federal Research Centre "Fundamentals of Biotechnology", Russian Academy of Sciences, Moscow, 119071, Russia. a.rojkova@fbras.ru.
Biochemistry. Biokhimiia
|April 8, 2021
Summary
This review covers CRISPR/CAS genome editing in filamentous fungi for industrial biotechnology. It highlights applications in improving fungal strains for producing valuable compounds like enzymes and metabolites.
Area of Science:
- Biotechnology
- Mycology
- Molecular Biology
Background:
- Filamentous fungi are crucial for industrial biotechnology, producing enzymes, metabolites, and other compounds.
- Genome editing offers a powerful tool to enhance the capabilities of these industrial fungal strains.
- The CRISPR/CAS system provides a precise and efficient method for modifying fungal genomes.
Purpose of the Study:
- To review the adaptation and application of the CRISPR/CAS system for genome editing in filamentous fungi.
- To present examples of CRISPR/CAS technology improving industrial fungal strains.
- To discuss the efficiency and optimization of CRISPR/CAS for specific industrial fungal producers.
Main Methods:
- Literature review of CRISPR/CAS system applications in filamentous fungi.
- Analysis of case studies involving industrial fungal strains (e.g., Trichoderma, Aspergillus, Penicillium).
- Evaluation of genome editing efficiency and system optimization strategies.
Main Results:
- CRISPR/CAS system is effectively adapted for genome editing in various filamentous fungi.
- Applications demonstrated improvements in industrial fungal strains for compound production.
- Specific examples showcase successful strain engineering using CRISPR/CAS technology.
Conclusions:
- CRISPR/CAS technology is a versatile tool for advancing industrial biotechnology through fungal strain improvement.
- Optimizing CRISPR/CAS systems is key to maximizing efficiency for specific industrial fungal applications.
- This review provides insights into the potential of genome editing for sustainable production of valuable compounds.
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