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Related Experiment Video

Updated: Nov 9, 2025

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
11:22

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EzMAP: Easy Microbiome Analysis Platform.

Gnanendra Shanmugam1, Song Hee Lee1,2, Junhyun Jeon3,4

  • 1Department of Biotechnology, Yeungnam University, Gyeongsan, 38541, Gyeongbuk, Korea.

BMC Bioinformatics
|April 8, 2021
PubMed
Summary

EzMAP is a user-friendly platform that simplifies microbiome data analysis from raw sequences to downstream applications. This tool integrates QIIME2 functionalities and offers streamlined analyses for researchers, especially beginners, in microbiome studies.

Keywords:
Microbiome analysis platformMicrobiome data analysisMicrobiome data visualizationMicrobiome user-friendly toolQIIME2 analysis

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Area of Science:

  • Microbiology
  • Bioinformatics
  • Computational Biology

Background:

  • Next-generation sequencing has advanced microbiome research, necessitating efficient computational pipelines.
  • Existing pipelines often require advanced programming skills and separate tools for downstream analysis.
  • There is a need for accessible platforms to process and analyze complex microbiome data.

Purpose of the Study:

  • To introduce EzMAP (Easy Microbiome Analysis Platform), a user-friendly graphical interface for microbiome data analysis.
  • To integrate QIIME2 functionalities with streamlined downstream analysis options.
  • To provide an accessible tool for researchers, particularly those new to microbiome data analysis.

Main Methods:

  • Developed using Java Swings, Java Script, and R programming language.
  • Standalone package with a graphical user interface (GUI).
  • Integrates QIIME2 for raw sequence processing and offers various downstream analysis modules.

Main Results:

  • EzMAP provides easy access to QIIME2 functionalities and facilitates downstream analyses.
  • Users can download processed data files like OTU tables, representative sequences, and phylogenetic trees.
  • Includes modules for relative abundance, community comparison (alpha/beta diversity), differential abundance, and functional prediction.

Conclusions:

  • EzMAP consolidates microbiome analysis from raw data to project-specific downstream analyses.
  • The platform is designed to be an invaluable tool for beginners in microbiome data analysis.
  • EzMAP is freely available and will be continuously updated.