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Response to correspondence on "Reproducibility of CRISPR-Cas9 methods for generation of conditional mouse alleles: a

Channabasavaiah B Gurumurthy1,2, Aidan R O'Brien3,4, Rolen M Quadros5

  • 1Mouse Genome Engineering Core Facility, Vice Chancellor for Research Office, University of Nebraska Medical Center, Omaha, NE, USA. cgurumurthy@unmc.edu.

Genome Biology
|April 8, 2021
PubMed
Abstract

No abstract available in PubMed .

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The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...

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