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Updated: Nov 9, 2025
Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma
Published on: April 12, 2019
Knockdown of MCM8 functions as a strategy to inhibit the development and progression of osteosarcoma through
Zhinan Ren1, Jun Li2, Shanwen Zhao3,4,5,6
1Department of Orthopedics, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China.
Abstract:
Osteosarcoma is the most common primary malignant tumor of bone derived from osteoblasts, which is a noteworthy threat to the health of children and adolescents. In this study, we found that MCM8 has significantly higher expression level in osteosarcoma tissues in comparison with normal tissues, which was also correlated with more advanced tumor grade and pathological stage. In agreement with the role of MCM proteins as indicators of cell proliferation, knockdown/overexpression of MCM8 inhibited/promoted osteosarcoma cell proliferation in vitro and tumor growth in vivo. Also, MCM8 knockdown/overexpression was also significantly associated with the promotion/inhibition of cell apoptosis and suppression/promotion of cell migration. More importantly, mechanistic study identified CTGF as a potential downstream target of MCM8, silencing of which could enhance the regulatory effects of MCM8 knockdown and alleviate the effects of MCM8 overexpression on osteosarcoma development. In summary, MCM8/CTGF axis was revealed as critical participant in the development and progression of osteosarcoma and MCM8 may be a promising therapeutic target for osteosarcoma treatment.
Insights
MCM8 is overexpressed in osteosarcoma, promoting tumor growth and cell migration. Targeting the MCM8/CTGF axis may offer a new therapeutic strategy for osteosarcoma treatment in children and adolescents.
Area of Science:
- Oncology
- Molecular Biology
- Biochemistry
Background:
- Osteosarcoma is a primary bone cancer affecting children and adolescents.
- MCM proteins are linked to cell proliferation, but their specific role in osteosarcoma is not fully understood.
Purpose of the Study:
- To investigate the role of MCM8 in osteosarcoma development and progression.
- To identify potential therapeutic targets for osteosarcoma.
Main Methods:
- Analysis of MCM8 expression in osteosarcoma tissues versus normal tissues.
- In vitro and in vivo experiments involving MCM8 knockdown and overexpression.
- Investigation of downstream targets, including CTGF.
Main Results:
- MCM8 expression is significantly higher in osteosarcoma and correlates with advanced tumor stage.
- MCM8 regulates osteosarcoma cell proliferation, apoptosis, and migration.
- The MCM8/CTGF axis plays a critical role in osteosarcoma progression.
Conclusions:
- MCM8 is a key driver in osteosarcoma.
- The MCM8/CTGF axis represents a promising therapeutic target for osteosarcoma treatment.
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