Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Computed Tomography01:10

Computed Tomography

7.5K
Tomography refers to imaging by sections. Computed tomography (CT) is a non-invasive imaging technique that uses computers to analyze several cross-sectional X-rays to reveal minute details about structures in the body.
The technique was invented in the 1970s and is based on the principle that as X-rays pass through the body, they are absorbed or reflected at different levels. In the technique, a patient lies on a motorized platform while a computerized axial tomography (CAT) scanner rotates...
7.5K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

11.6K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
11.6K
Distance Corrections01:15

Distance Corrections

138
To achieve precise distance measurements, especially in surveying and construction, certain corrections must be applied to account for potential sources of error like the standardization errors, temperature variations, and slope adjustments.Standardization error emerges when measurement equipment undergoes changes, such as wear, repairs, or weather impacts. To address this, surveyors compare the equipment’s readings to a standard. This process identifies any deviation that might lead to...
138
X-ray Imaging01:24

X-ray Imaging

9.2K
German physicist Wilhelm Röntgen (1845–1923) was experimenting with electrical current when he discovered that a mysterious and invisible "ray" would pass through his flesh but leave an outline of his bones on a screen coated with a metal compound. In 1895, Röntgen made the first durable record of the internal parts of a living human: an "X-ray" image (as it came to be called) of his wife’s hand. Scientists worldwide quickly began their own experiments with...
9.2K
NMR Spectrometers: Resolution and Error Correction01:14

NMR Spectrometers: Resolution and Error Correction

898
When magnetic nuclei in a sample achieve resonance and undergo relaxation, the signal detected in NMR is an approximately exponential free induction decay. Fourier transform of an exponential decay yields a Lorentzian peak in the frequency domain. Lorentzian peaks in an NMR spectrum are defined by their amplitude, full width at half maximum, and position, where the peak width is governed by the spin-spin relaxation time alone. In real experiments, however, the applied magnetic field is rendered...
898
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

11.3K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
11.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Transcription and cohesin direct domain boundary spatial positioning and are linked to Friedreich's ataxia.

Molecular cell·2026
Same author

Harnessing Methyltransferase-Guided Targeting for Sequence-Specific Proximity Labeling of DNA.

Angewandte Chemie (International ed. in English)·2026
Same author

KRAS Can Bind to FTase Despite Disruption of the CAAX Binding Site.

Biochemistry·2026
Same author

FLIPs: Genetically encoded molecular biosensors for functional imaging of cell signaling by linear dichroism microscopy.

Science advances·2026
Same author

Morphological and Hyperphosphorylation Transitions of Nanoscale Tau Aggregates in Alzheimer's Disease.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2025
Same author

Super-resolved Imaging of Molecular Interactions Using FRET-SOFI.

Nano letters·2025
Same journal

A tri-axis optomechanical accelerometer with plasmonic MIM waveguide and structural direction-dependent optical signatures.

Scientific reports·2026
Same journal

Holographic leaky-wave antennas with independently controlled multiple counter-rotating vortex beams.

Scientific reports·2026
Same journal

Differential associations of longitudinal hearing and vision trajectories with dementia and mild cognitive impairment in older adults.

Scientific reports·2026
Same journal

Abdominal obesity and leisure-time sedentary behavior in relation to gastroesophageal reflux disease risk: a prospective cohort study from the UK Biobank.

Scientific reports·2026
Same journal

Effect of nitrogen-rich COF incorporation on the structure and separation performance of polyamide nanofiltration membranes.

Scientific reports·2026
Same journal

Withanolide A inhibits hIAPP aggregation: An In silico, biophysical, and drosophila-based In vivo validation.

Scientific reports·2026
See all related articles

Related Experiment Video

Updated: Nov 9, 2025

Sample Drift Correction Following 4D Confocal Time-lapse Imaging
10:04

Sample Drift Correction Following 4D Confocal Time-lapse Imaging

Published on: April 12, 2014

16.7K

Smoothness correction for better SOFI imaging.

Siewert Hugelier1, Wim Vandenberg2, Tomáš Lukeš3

  • 1Laboratory for Nanobiology, KU Leuven, 3001, Leuven, Belgium. siewert.hugelier@gmail.com.

Scientific Reports
|April 8, 2021
PubMed
Summary
This summary is machine-generated.

We developed a new method using Whittaker smoothing to improve super-resolution optical fluctuation imaging (SOFI). This technique enhances image contrast and detail by effectively removing noise from processes like photodestruction.

More Related Videos

High-Accuracy Correction of 3D Chromatic Shifts in the Age of Super-Resolution Biological Imaging Using Chromagnon
08:18

High-Accuracy Correction of 3D Chromatic Shifts in the Age of Super-Resolution Biological Imaging Using Chromagnon

Published on: June 16, 2020

7.7K
Quantifying Intermembrane Distances with Serial Image Dilations
07:45

Quantifying Intermembrane Distances with Serial Image Dilations

Published on: September 28, 2018

6.6K

Related Experiment Videos

Last Updated: Nov 9, 2025

Sample Drift Correction Following 4D Confocal Time-lapse Imaging
10:04

Sample Drift Correction Following 4D Confocal Time-lapse Imaging

Published on: April 12, 2014

16.7K
High-Accuracy Correction of 3D Chromatic Shifts in the Age of Super-Resolution Biological Imaging Using Chromagnon
08:18

High-Accuracy Correction of 3D Chromatic Shifts in the Age of Super-Resolution Biological Imaging Using Chromagnon

Published on: June 16, 2020

7.7K
Quantifying Intermembrane Distances with Serial Image Dilations
07:45

Quantifying Intermembrane Distances with Serial Image Dilations

Published on: September 28, 2018

6.6K

Area of Science:

  • Biophysics
  • Optical Imaging
  • Microscopy

Background:

  • Super-resolution fluorescence imaging visualizes cellular structures at the nanoscale.
  • Super-resolution optical fluctuation imaging (SOFI) enhances resolution by analyzing fluorophore blinking.
  • SOFI can be negatively impacted by non-stationary processes like photodestruction and illumination fluctuations.

Purpose of the Study:

  • To introduce Whittaker smoothing as a method to improve SOFI signal quality.
  • To effectively remove smooth signal trends caused by non-stationary processes.
  • To enhance the contrast and detail in super-resolution microscopy images.

Main Methods:

  • Application of Whittaker smoothing to filter out signal trends in SOFI data.
  • Analysis of fluorophore blinking dynamics to improve spatial resolution.
  • Parameter-free and computationally efficient signal processing technique.

Main Results:

  • Whittaker smoothing effectively corrects for photodestruction, particularly rapid degradation.
  • Resulting SOFI images exhibit significantly higher contrast and suppressed background noise.
  • Improved visualization of cellular structures with enhanced nanoscale detail.

Conclusions:

  • Whittaker smoothing is a robust and efficient method for enhancing SOFI data.
  • The technique improves image quality by isolating emitter blinking signals.
  • Applicable to both 2D and 3D super-resolution imaging for detailed cellular analysis.