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Updated: Nov 9, 2025

Novel Sequence Discovery by Subtractive Genomics
Published on: January 25, 2019
The complete chloroplast genome of Castanea sativa Mill. (Fagaceae)
Cancan Zhu1, Shijie Zhang1, Xiaoqian Bai1
1Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing, PR China.
Abstract:
Castanea sativa Mill. is mainly grown in the temperate regions of continental Europe, and it has a considerable economic value. In this study, the complete chloroplast genome sequence of Castanea sativa was characterized. Leaves were collected from the National Botanical Garden of Latvia. The chloroplast genome was determined to be 160,938 bp in length. It contained large single-copy (LSC) and small single-copy (SSC) regions of 90,519 and 18,967 bp, respectively, which were separated by a pair of 25,726 bp inverted repeat (IR) regions. The genome is predicted to contain 130 genes, including 83 protein-coding genes, 37 tRNA genes, eight rRNA genes, and two pseudo genes. The overall GC content of the genome is 36.8%. A phylogenetic tree reconstructed by 34 chloroplast genomes reveals that C. sativa is most closely related to the clade including C. henryi, C. seguinii and C. mollissima.
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