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Related Experiment Video

Updated: Nov 9, 2025

Exploring the Root Microbiome: Extracting Bacterial Community Data from the Soil, Rhizosphere, and Root Endosphere
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Microbiomes in forensic botany: a review.

Sarah Ishak1, Eleanor Dormontt2, Jennifer M Young3

  • 1Département de Biologie, Faculté des Sciences, Université de Sherbrooke, Sherbrooke, Quebec, Canada. ishs3601@usherbrooke.ca.

Forensic Science, Medicine, and Pathology
|April 8, 2021
PubMed
Summary
This summary is machine-generated.

Forensic botanical analysis can be enhanced by studying plant microbiomes. This approach offers greater potential for identifying crime scene locations using plant fragments.

Keywords:
Forensic ScienceMetabarcodingMetagenomicsMicrobiomesPlantsProvenance

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Area of Science:

  • Forensic Science
  • Microbiology
  • Botany

Background:

  • Botanical fragments at crime scenes offer valuable circumstantial evidence.
  • Current morphological and genetic analyses have limitations in pinpointing local source locations.

Purpose of the Study:

  • To review current methods for analyzing botanical fragments in forensic investigations.
  • To introduce microbiome analysis as a complementary tool for plant fragment analysis.
  • To explore the potential of phyllosphere microbiome for identifying plant fragment provenance.

Main Methods:

  • Review of existing forensic botanical analysis techniques.
  • Introduction of microbiome analysis of the phyllosphere.
  • Identification of key research areas for developing microbiome analysis tools.

Main Results:

  • Morphological and genetic analyses are limited in differentiating local source locations.
  • Phyllosphere microbiome analysis shows potential for enhanced provenance identification.
  • Further research is needed on microbial DNA retrieval, microbiome variability, and environmental impacts.

Conclusions:

  • Microbiome analysis of plant fragments can significantly improve the identification of crime scene locations.
  • Development of robust microbiome analysis tools is crucial for forensic botany.
  • This approach can increase the evidentiary value of botanical evidence in casework.