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Author Spotlight: Advancing the Analysis of Plasma Extracellular Vesicle Proteome for Cardiovascular Biomarker Studies
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Protein profiles in plasma: Development from infancy to 5 years of age

Maria Mikus1,2, Hans Järnbert-Pettersson3, Catharina Johansson3

  • 1Department of Protein Science, Division of Affinity Proteomics, SciLifeLab, KTH Royal Institute of Technology, Stockholm, Sweden.

Proteomics. Clinical Applications
|April 8, 2021
PubMed

Insights

Plasma protein levels change significantly throughout early childhood, with many alterations occurring regardless of lifestyle. This study tracked 97 proteins in children across different lifestyles to understand early life proteome development.

Area of Science:

  • Pediatric Proteomics
  • Developmental Biology
  • Lifestyle Medicine

Background:

  • Longitudinal data on plasma protein development in early childhood is limited.
  • The influence of lifestyle factors on early-life plasma proteome dynamics remains underexplored.

Purpose of the Study:

  • To longitudinally monitor plasma protein levels in early childhood.
  • To investigate the impact of different lifestyles (anthroposophic, partly anthroposophic, non-anthroposophic) on plasma proteome development.

Main Methods:

  • Analyzed plasma levels of 97 proteins using multiplex bead-based immunoassay.
  • Collected 280 blood samples longitudinally from children at 6, 12, 24, and 60 months of age.
  • Stratified analysis based on three distinct family lifestyle groups.

Main Results:

  • 68 out of 97 (70%) proteins showed significant changes between 6 months and 5 years of age.
  • 59% of proteins altered over time within at least one lifestyle group.
  • Four distinct longitudinal trends in plasma protein levels were identified, varying by lifestyle.

Conclusions:

  • Plasma proteome undergoes significant longitudinal development in early childhood.
  • Lifestyle factors influence, but do not entirely dictate, these developmental changes.
  • Findings enhance understanding of early-life proteome trajectories and lifestyle interactions.
Abstract

Keywords:
ALADDINchildhoodlifestylelongitudinal

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