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SK-UT-1B, a human tumorigenic diploid cell line
1American Type Culture Collection, Rockville, Maryland 20852.
Cancer Genetics and Cytogenetics
|July 1, 1988
Summary
Human solid tumor cells (SK-UT-1B) were proven tumorigenic in mice. Despite tumor formation, detailed karyotypic analysis revealed no chromosomal abnormalities, suggesting a diploid karyotype in these cancer cells.
Area of Science:
- Oncology
- Cell Biology
- Genetics
Background:
- The SK-UT-1B cell line is derived from a human solid tumor.
- Tumorigenicity is a key characteristic for cancer cell line models.
- Maintaining a stable karyotype is important for reproducible experimental results.
Purpose of the Study:
- To assess the tumorigenic potential of the SK-UT-1B cell line in vivo.
- To investigate the chromosomal stability of the SK-UT-1B cell line after demonstrating tumorigenicity.
Main Methods:
- Subcutaneous injection of 10 million SK-UT-1B cells into athymic nude mice.
- Tumor formation was monitored over two weeks.
- Karyotypic analysis was performed at the 500-600 subband level.
Main Results:
- SK-UT-1B cells successfully produced tumors within two weeks post-injection.
- Detailed karyotypic analysis showed no noticeable chromosomal aberrations.
- The cell line maintained a diploid karyotype despite in vivo tumor formation.
Conclusions:
- The SK-UT-1B cell line is a tumorigenic model of human solid cancer.
- This cell line represents a rare example of experimentally validated human tumor cells with a stable diploid karyotype.
- It offers a unique model for studying tumorigenesis without confounding chromosomal abnormalities.