Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Varying oncomodulin mRNA abundance in developing placenta and solid tumors.

M F Gillen1, L M Brewer, J P MacManus

  • 1Division of Biological Sciences, National Research Council of Canada, Ottawa.

Cancer Letters
|June 15, 1988
PubMed
Summary

Researchers developed a sensitive antisense RNA probe to detect oncomodulin mRNA in rat tumors and placenta. This probe revealed a direct relationship between oncomodulin mRNA and protein levels, suggesting transcriptional regulation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Isoflurane effects on the N1 and other long-latency auditory evoked potentials in Wistar rats.

Neuroscience research·2021
Same author

Effects of metal ion binding on an oncomodulin mutant containing a novel calcium-binding loop.

Journal of fluorescence·2013
Same author

Measurement of functional residual capacity by modified multiple breath nitrogen washout for spontaneously breathing and mechanically ventilated patients.

British journal of anaesthesia·2011
Same author

The transcription factor E2F1 promotes dopamine-evoked neuronal apoptosis by a mechanism independent of transcriptional activation.

Journal of neurochemistry·2001
Same author

Attenuation of neurotoxicity in cortical cultures and hippocampal slices from E2F1 knockout mice.

Journal of neurochemistry·2001
Same author

Increased expression of the transcription factor E2F1 during dopamine-evoked, caspase-3-mediated apoptosis in rat cortical neurons.

Neuroscience letters·2001

Area of Science:

  • Molecular Biology
  • Oncology
  • Developmental Biology

Background:

  • Oncomodulin is a protein implicated in tumor growth and development.
  • Accurate quantification of oncomodulin mRNA is crucial for understanding its role in various biological processes.

Purpose of the Study:

  • To develop a sensitive and specific method for detecting and quantifying oncomodulin mRNA in rat tumors and placenta.
  • To investigate the relationship between oncomodulin mRNA and protein levels in these tissues.

Main Methods:

  • Preparation of an antisense RNA probe complementary to rat oncomodulin mRNA using run-off transcription.
  • RNA isolation from chemically-induced hepatoma and virally transformed sarcomas, as well as rat placenta.
  • Detection and size characterization of oncomodulin mRNA using Northern blot analysis.

Related Experiment Videos

  • Quantitative analysis of oncomodulin mRNA levels via dot blot autoradiography and densitometry.

    • Main Results:

    • A common hybridizable RNA of approximately 750 nucleotides, corresponding to oncomodulin mRNA, was identified in both tumor tissues and placenta.
    • The antisense RNA probe enabled sensitive detection of oncomodulin mRNA in developing placenta, a feat not achievable with previous DNA probes.
    • Quantitative measurements revealed a direct correlation between oncomodulin mRNA and protein levels in both tumor and placental tissues.

    Conclusions:

    • The developed antisense RNA probe is a valuable tool for sensitive detection and quantification of oncomodulin mRNA.
    • Oncomodulin abundance in tumors and placenta appears to be primarily regulated at the transcriptional level.
    • This study provides insights into the molecular mechanisms governing oncomodulin expression in cancer and development.