Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Glucose diffusivity in multicellular tumor spheroids.

J J Casciari1, S V Sotirchos, R M Sutherland

  • 1Experimental Therapeutics Division, University of Rochester Cancer Center, NY 14627.

Cancer Research
|July 15, 1988
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Shutdown of immunological priming and presentation after in vivo administration of adenovirus.

Gene therapy·2011
Same author

Without CD4 help, CD8 rejection of pig xenografts requires CD28 costimulation but not perforin killing.

Journal of immunology (Baltimore, Md. : 1950)·2001
Same author

Cytotoxicity of ascorbate, lipoic acid, and other antioxidants in hollow fibre in vitro tumours.

British journal of cancer·2001
Same author

Cell-associated ovalbumin is cross-presented much more efficiently than soluble ovalbumin in vivo.

Journal of immunology (Baltimore, Md. : 1950)·2001
Same author

Overcoming the poor immunogenicity of a protein by DNA immunization as a fusion construct.

Immunology and cell biology·2001
Same author

Delayed rejection of fetal pig pancreas in CD4 cell deficient mice was correlated with residual helper activity.

Xenotransplantation·2000
Same journal

SEPTIN7 Enhances Tumorgenesis and Therapeutic Resistance in Hepatocellular Carcinoma by Promoting FGFR4 Stabilization and Recycling.

Cancer research·2026
Same journal

TLR9 Agonists Potentiate Adoptive T Cell Therapy in Cancer through a B Cell-CD2 Costimulatory Axis.

Cancer research·2026
Same journal

CDK2 Inhibition Exerts RB-Independent Antitumor Activity in CDK4/6 Inhibitor-Resistant HR+/HER2- Breast Cancer.

Cancer research·2026
Same journal

A Clinically Integrated Pediatric Patient-Derived Xenograft Program Enables Evaluation of Cohort and Patient-Specific Biology and Therapeutic Strategies.

Cancer research·2026
Same journal

Editor's Note: Heterodimerization of Insulin-like Growth Factor Receptor/Epidermal Growth Factor Receptor and Induction of Survivin Expression Counteract the Antitumor Action of Erlotinib.

Cancer research·2026
Same journal

Editor's Note: Deguelin Analogue SH-1242 Inhibits Hsp90 Activity and Exerts Potent Anticancer Efficacy with Limited Neurotoxicity.

Cancer research·2026
See all related articles

Understanding glucose diffusion in tumors is key for cancer research. This study measured glucose diffusivity in multicellular tumor spheroids, revealing values low enough to suggest significant glucose gradients within tumors, impacting growth and heterogeneity.

Area of Science:

  • Oncology
  • Biophysics
  • Biochemistry

Background:

  • Multicellular tumor spheroids (MCTSs) are used as models for solid tumors.
  • Glucose availability is a critical factor influencing tumor growth and cellular heterogeneity.
  • Accurate measurement of glucose diffusion is essential for understanding these limitations.

Purpose of the Study:

  • To determine the effective diffusion coefficient of glucose in various tumor spheroids.
  • To investigate the implications of glucose diffusion rates on spheroid growth and cellular characteristics.

Main Methods:

  • Utilized tritium-labeled L-glucose as a diffusion tracer to measure efflux from spheroids.
  • Applied the diffusion equation, accounting for L-glucose binding, to calculate effective glucose diffusivity.

Related Experiment Videos

  • Conducted experiments on EMT6/Ro mouse mammary tumor spheroids and human tumor cell lines (HT29, CO112, WiDr, CaSki, A431).

    • Main Results:

    • Effective glucose diffusivity in EMT6/Ro spheroids was measured at 1.1 x 10^-6 cm²/s.
    • Glucose diffusion coefficients in human tumor spheroids ranged from 5.5 x 10^-7 cm²/s to 2.3 x 10^-7 cm²/s.
    • These diffusion values are sufficiently low to indicate potential glucose concentration gradients within spheroids and tumors.

    Conclusions:

    • The determined glucose diffusivities suggest that glucose limitations may significantly influence spheroid and tumor growth.
    • Variations in glucose diffusion coefficients across different cell lines highlight cell-specific factors affecting nutrient transport.
    • These findings have implications for understanding tumor cell heterogeneity and developing targeted therapies.