Related Experiment Video

Updated: Nov 9, 2025

Author Spotlight: Unveiling the Role of TMOD3 in Platinum Resistance and Immune Infiltration in Ovarian Cancer
09:40

Author Spotlight: Unveiling the Role of TMOD3 in Platinum Resistance and Immune Infiltration in Ovarian Cancer

Published on: August 2, 2024

2.9K

Oncogenic role of MiR-130a in oral squamous cell carcinoma

Karthik Mallela1, Swamy Shivananda2, Kodaganur S Gopinath2

  • 1Department of Molecular Reproduction, Development and Genetics, Indian Institute of Science, Bangalore, 560012, India.

Scientific Reports
|April 9, 2021
PubMed

Insights

Oncogenic miR-130a promotes oral cancer by downregulating TSC1. Restoring TSC1 levels with antagomiR-130a offers a potential therapeutic strategy for oral squamous cell carcinoma (OSCC).

Area of Science:

  • Molecular Oncology
  • Cancer Biology
  • MicroRNA Therapeutics

Background:

  • Aberrant PI3K/AKT/mTOR pathway activation drives oral squamous cell carcinoma (OSCC) pathogenesis.
  • MicroRNAs (miRNAs) are implicated in oral carcinogenesis, acting as tumor suppressors or oncogenes.
  • TSC1, a pathway component, regulates cell proliferation, apoptosis, migration, and invasion; its downregulation correlates with poor prognosis in OSCC.

Purpose of the Study:

  • To investigate the role of miR-130a in regulating TSC1 expression in OSCC.
  • To elucidate the functional consequences of miR-130a-mediated TSC1 repression on OSCC cell behavior.
  • To evaluate antagomiR-130a as a potential therapeutic agent for OSCC.

Main Methods:

  • Luciferase reporter assays to confirm miR-130a binding to the TSC1 3'UTR.
  • In vitro assays assessing cell proliferation, anchorage-independent growth, and invasion.
  • Analysis of miR-130a and TSC1 expression correlation in OSCC patient samples.
  • In vivo xenograft studies using antagomiR-130a in OSCC models.

Main Results:

  • Oncogenic miR-130a directly binds to and represses TSC1 expression in OSCC cells.
  • MiR-130a-induced TSC1 downregulation enhances OSCC cell proliferation, anchorage-independent growth, and invasion.
  • An inverse correlation between miR-130a and TSC1 levels was observed in OSCC samples.
  • AntagomiR-130a treatment significantly reduced OSCC xenograft tumor size.

Conclusions:

  • MiR-130a-mediated downregulation of TSC1 is a novel mechanism contributing to OSCC development.
  • Restoration of TSC1 levels via antagomiR-130a represents a promising therapeutic strategy for OSCC.
  • Targeting the miR-130a/TSC1 axis holds potential for oral cancer treatment.

More Related Videos

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis11:44

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

7.8K
Intramucosal Inoculation of Squamous Cell Carcinoma Cells in Mice for Tumor Immune Profiling and Treatment Response Assessment07:29

Intramucosal Inoculation of Squamous Cell Carcinoma Cells in Mice for Tumor Immune Profiling and Treatment Response Assessment

Published on: April 22, 2019

11.8K

Related Concept Videos

Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
4.8K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.8K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.4K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
22.8K
The Retinoblastoma Gene01:20

The Retinoblastoma Gene

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
4.3K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
4.0K