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Related Concept Videos

Spectrophotometry: Introduction01:16

Spectrophotometry: Introduction

5.8K
Spectrophotometry is the quantitative measurement of the absorption, reflection, diffraction, or transmission of electromagnetic radiation through a material as a function of the intensity and wavelength of the radiation. A spectrophotometer is a device used to measure the change in the radiation intensity caused by its interaction with the material.
The essential components of a spectrophotometer include a source of electromagnetic radiation, a slot for placing a material to be analyzed, and a...
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Applications of IR Spectroscopy: Overview01:11

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The non-destructive nature and ability to provide valuable chemical information make IR spectroscopy a versatile technique with broad applications in various scientific and industrial fields. IR spectroscopy is commonly used to identify and characterize organic and inorganic compounds. It provides information about the functional groups present in a molecule and the bonding between atoms. This helps in the structural elucidation of compounds during organic synthesis, pharmaceutical research,...
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Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview01:13

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Attenuated total reflectance (ATR) infrared spectroscopy is a powerful analytical technique used to study the composition of materials. It is widely employed in chemistry, materials science, forensic science, and other fields where sample characterization is required. ATR has several advantages over traditional transmission IR spectroscopy, including the requirement of little to no sample preparation and the ability to analyze a wide range of samples.
The ATR process begins by directing a beam...
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IR Spectrometers01:25

IR Spectrometers

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There are two main infrared (IR) spectrophotometers: dispersive IR spectrometers and Fourier transform infrared (FTIR) spectrometers. In a dispersive IR spectrometer, a beam of infrared radiation produced by a hot wire is divided into two parallel equal-intensity beams using mirrors. One beam passes through the sample, while another is a reference beam. The beams then move through the monochromator, which separates the radiations into a continuous spectrum of different frequencies. The...
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UV–Vis Spectrometers01:14

UV–Vis Spectrometers

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The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell.
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Related Experiment Video

Updated: Nov 9, 2025

Multimodal Imaging and Spectroscopy Fiber-bundle Microendoscopy Platform for Non-invasive, In Vivo Tissue Analysis
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Spectral Reflectometry in Biomedical Imaging and Sensing.

Junhwan Kwon1, Yongjae Jo1, Myunghwan Choi2

  • 1Department of Biomedical Engineering, Sungkyunkwan University, Suwon, South Korea.

Advances in Experimental Medicine and Biology
|April 9, 2021
PubMed
Summary

Spectral reflectometry, a nanoscale measurement technique, is now advancing biomedicine. This method uses thin-film interference for applications ranging from molecular sensing to biomedical imaging.

Keywords:
AxonLive microscopyMyelinOptical probeReflectophoreSpectral reflectometry

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Area of Science:

  • Biomedical Optics
  • Nanotechnology
  • Spectroscopy

Background:

  • Spectral reflectometry is a spectroscopic technique utilizing thin-film interference for nanoscale measurements.
  • It has been extensively used in industries for precise thickness measurements of dielectric layers.
  • Recent progress in biological nanostructures has spurred its application in biomedicine.

Purpose of the Study:

  • To provide a comprehensive overview of spectral reflectometry in the biomedical field.
  • To cover the fundamental principles of the technique.
  • To explore its diverse applications in medical research and diagnostics.

Main Methods:

  • The chapter details the principles of spectral reflectometry based on thin-film interference.
  • It discusses the adaptation of this technique for biological samples.
  • Methods for molecular sensing and biomedical imaging are explained.

Main Results:

  • Spectral reflectometry offers a non-invasive method for analyzing biological nanostructures.
  • The technique enables molecular-level sensing with high sensitivity.
  • Applications in biomedical imaging provide nanoscale resolution.

Conclusions:

  • Spectral reflectometry is a powerful tool with significant potential in biomedicine.
  • Its application extends from fundamental research to clinical diagnostics.
  • Further development promises enhanced capabilities in medical sensing and imaging.