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Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA
Published on: July 9, 2021
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Functional annotation of lncRNA in high-throughput screening
Chi Wai Yip1, Divya M Sivaraman1,2, Anika V Prabhu1
1RIKEN Center for Integrative Medical Sciences, Yokohama, Kanagawa 230-0045, Japan.
Essays in Biochemistry
|April 9, 2021
Summary
Functional roles of long non-coding RNAs (lncRNAs) in cellular processes and diseases are increasingly recognized. High-throughput screening methods are crucial for identifying the functions of the vast majority of unknown lncRNAs.
Area of Science:
- Molecular Biology
- Genomics
- Biochemistry
Background:
- Long non-coding RNAs (lncRNAs) are key regulators of diverse cellular processes, including pluripotency, lineage commitment, and disease pathogenesis.
- Despite the discovery of over 173,000 lncRNAs, the majority remain functionally uncharacterized.
- Cell type-specific expression patterns suggest distinct functional roles for lncRNAs in different cellular contexts.
Purpose of the Study:
- To review current high-throughput (HTP) screening technologies and strategies for identifying functional lncRNAs.
- To discuss the application of HTP screening in functional annotation of lncRNAs across various biological processes and diseases.
- To provide insights into selecting appropriate technologies for efficient lncRNA functional characterization.
Main Methods:
- Review of existing literature on high-throughput screening methodologies for lncRNA functional analysis.
- Discussion of diverse technologies, screening platforms, and cellular responses employed in lncRNA research.
- Analysis of downstream data processing and interpretation techniques for HTP screening results.
Main Results:
- lncRNAs interact with DNA, RNA, and proteins to regulate fundamental cellular mechanisms like transcription, RNA processing, and translation.
- HTP screening approaches are essential for deciphering the functions of the large number of unannotated lncRNAs.
- Various technologies and platforms are available for HTP screening, each with specific advantages for lncRNA functional annotation.
Conclusions:
- Identifying functional lncRNAs through HTP screening is critical for understanding their roles in biological processes and diseases.
- The choice of screening technology and platform significantly impacts the efficiency of lncRNA functional annotation.
- This review aims to guide researchers in applying suitable HTP screening strategies for comprehensive lncRNA functional characterization.
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