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Related Concept Videos

Alternative RNA Splicing02:18

Alternative RNA Splicing

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Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
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Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
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lncRNA - Long Non-coding RNAs02:39

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In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
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Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
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Using the E1A Minigene Tool to Study mRNA Splicing Changes
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Long non-coding RNAs and splicing.

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  • 1Institute of Molecular Genetics, Czech Academy of Sciences, Prague, Czech Republic.

Essays in Biochemistry
|April 9, 2021
PubMed
Summary
This summary is machine-generated.

Splicing efficiency differs between protein-coding genes and long non-coding RNAs (lncRNAs). This review explores lncRNA splicing, conservation, and its role in cellular protection from aberrant transcripts.

Keywords:
SR proteinslarge intervening non-coding RNAsnRNPspliceosomessplicing

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Area of Science:

  • Molecular Biology
  • Genetics
  • RNA Biology

Background:

  • Long non-coding RNAs (lncRNAs) are crucial regulatory molecules.
  • Splicing is a key post-transcriptional modification process.
  • Understanding lncRNA processing is vital for comprehending gene regulation.

Purpose of the Study:

  • To review the distinct splicing efficiencies of lncRNAs compared to protein-coding genes.
  • To explore the evolutionary conservation of splice sites in lncRNAs.
  • To investigate potential novel roles of splicing in lncRNA metabolism and cellular defense.

Main Methods:

  • Literature review and synthesis of existing research on RNA splicing.
  • Comparative analysis of splicing patterns in lncRNAs and protein-coding transcripts.
  • Hypothesis generation based on current understanding of splicing machinery and lncRNA function.

Main Results:

  • lncRNAs exhibit generally lower splicing efficiency than protein-coding genes.
  • Splice sites represent highly conserved regions within lncRNAs.
  • Splicing machinery may play a protective role against unwanted transcripts.

Conclusions:

  • Splicing dynamics in lncRNAs are unique and warrant further investigation.
  • Conserved splice sites suggest functional importance beyond simple intron removal.
  • The splicing machinery might act as a cellular safeguard for lncRNA integrity.