Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA Editing02:23

RNA Editing

9.4K
RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
9.4K
RACE - Rapid Amplification of cDNA Ends02:35

RACE - Rapid Amplification of cDNA Ends

6.7K
Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific...
6.7K
Ribosome Profiling02:24

Ribosome Profiling

3.8K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.8K
RNA Structure01:23

RNA Structure

76.6K
Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
76.6K
Types of RNA01:20

Types of RNA

8.0K
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
8.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Rational design of mechanically active RNAs: de novo engineering of functional exoribonuclease-resistant RNAs.

Nucleic acids research·2026
Same author

Spark: sparse hierarchical energy minimization for scalable prediction of RNA pseudoknots.

Bioinformatics (Oxford, England)·2026
Same author

Observation of self-bound droplets of ultracold dipolar molecules.

Nature·2026
Same author

Undesignable motifs in structural RNAs and combinatorial consequences.

Journal of mathematical biology·2026
Same author

Trapping of single atoms in metasurface optical tweezer arrays.

Nature·2026
Same author

Designing molecular RNA switches with Restricted Boltzmann machines.

Nature communications·2025

Related Experiment Video

Updated: Nov 9, 2025

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells
10:34

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

Published on: December 9, 2022

4.7K

Advanced Design of Structural RNAs Using RNARedPrint.

Yann Ponty1, Stefan Hammer2,3, Hua-Ting Yao4,5

  • 1LIX, CNRS UMR 7161, Ecole Polytechnique, Institut Polytechnique de Paris, Palaiseau, France. yann.ponty@lix.polytechnique.fr.

Methods in Molecular Biology (Clifton, N.J.)
|April 9, 2021
PubMed
Summary
This summary is machine-generated.

This study introduces RNARedPrint, a novel software tool for the de novo rational design of RNA sequences. It enables the creation of RNA molecules with specific secondary structures for biotechnological applications.

Keywords:
Kinetic landscapesRNA designRiboswitches

More Related Videos

Use of Alu Element Containing Minigenes to Analyze Circular RNAs
13:10

Use of Alu Element Containing Minigenes to Analyze Circular RNAs

Published on: March 10, 2020

7.5K
RNA Secondary Structure Prediction Using High-throughput SHAPE
13:42

RNA Secondary Structure Prediction Using High-throughput SHAPE

Published on: May 31, 2013

31.8K

Related Experiment Videos

Last Updated: Nov 9, 2025

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells
10:34

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

Published on: December 9, 2022

4.7K
Use of Alu Element Containing Minigenes to Analyze Circular RNAs
13:10

Use of Alu Element Containing Minigenes to Analyze Circular RNAs

Published on: March 10, 2020

7.5K
RNA Secondary Structure Prediction Using High-throughput SHAPE
13:42

RNA Secondary Structure Prediction Using High-throughput SHAPE

Published on: May 31, 2013

31.8K

Area of Science:

  • Molecular Biology
  • Synthetic Biology
  • Computational Biology

Background:

  • Designing novel RNA molecules with specific functions is crucial for synthetic biology and biotechnology.
  • Existing RNA design methods face challenges in generating desired secondary structures and maintaining realistic base composition.

Purpose of the Study:

  • To present RNARedPrint, a software tool for the de novo rational design of RNA sequences.
  • To enable the design of RNA molecules with one or several desired secondary structures, including alternate configurations or kinetic pathways.

Main Methods:

  • Utilizing the RNARedPrint software for computational RNA sequence design.
  • Focusing on rational design principles to achieve specific RNA secondary structures.

Main Results:

  • RNARedPrint facilitates convenient and rapid de novo RNA sequence design.
  • The software effectively overcomes common RNA design challenges, such as maintaining realistic GC content.

Conclusions:

  • RNARedPrint offers a practical solution for routine RNA design.
  • The tool supports the creation of novel RNA molecules with predictable structures for diverse applications.