Efficient development of silk fibroin membranes on liquid surface for potential use in biomedical materials

Silk fibroin (SF) protein is versatile for the application of biomaterials due to its excellent mechanical properties, biocompatibility and biodegradability. However, the efficient way to fabricate SF membranes with special structure is still challenging. Here, we develop an efficient and simple way to create SF membranes on the liquid (i.e. subphase) surface. It is essential to prepare highly concentrated SF solution with low surface tension by dissolving the degummed SF powders in 6% (w/v) LiBr/methanol solution by one step. 95 wt% polyethylene glycol (PEG) 200 and 30 wt% (NH₄)₂SO₄ are the subphases, on which the SF solution spreads quickly, generating nonporous and microporous SF membranes (SFM-1 and SFM-2), respectively. PEG 200 causes more ordered molecular packing (β-sheets) in SFM-1. While Fast diffusion and denaturation of SF on (NH₄)₂SO₄ solution lead to the formation of microporous, water-unstable membrane SFM-2. Both membranes have good transparency, hydrophilicty, and mechanical properties. To fabricate antibacterial biomaterials, we design a composite membrane by SFM-1 and SFM-2 sandwiching a layer of hydroxypropyl trimethylammonium chloride chitosan (HACC) to provide antibacterial functions. The sandwich membrane has good cell viability and antibacterial properties, showing potential use for biomedical materials.