Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Master Transcription Regulators02:23

Master Transcription Regulators

7.3K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
7.3K
Stem Cell Niche01:26

Stem Cell Niche

5.7K
The stem cell niche is the dynamic microenvironment where stem cells reside. Inside these niches, the cells may remain undifferentiated, undergo high self-renewal, or become lineage-specific progenitors. Stem cells coexist with other niche cells, such as stromal cells. They also interact closely with the ECM. Cell-cell and cell-matrix communication occur via adhesion molecules or soluble factors that signal the stem cells and determine their fate. Stromal cells also provide survival signals to...
5.7K
Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

2.4K
Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
2.4K
Maintenance of the ES Cell State01:14

Maintenance of the ES Cell State

2.4K
The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...
2.4K
Combinatorial Gene Control02:33

Combinatorial Gene Control

8.8K
Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
8.8K
General Transcription Factors01:30

General Transcription Factors

6.2K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
6.2K
  1. Home
  3. Transcriptional Networks Controlling Stromal Cell Differentiation.
  1. Home
  3. Transcriptional Networks Controlling Stromal Cell Differentiation.

Related Experiment Video

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes
10:48

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes

Published on: April 12, 2015

10.3K

Transcriptional networks controlling stromal cell differentiation.

Alexander Rauch1,2, Susanne Mandrup3

  • 1Molecular Endocrinology & Stem Cell Research Unit (KMEB), Department of Endocrinology and Metabolism, Odense University Hospital and Department of Clinical Research, University of Southern Denmark, Odense, Denmark. arauch@health.sdu.dk.

Nature Reviews. Molecular Cell Biology
|April 10, 2021

View abstract on PubMed

Summary
This summary is machine-generated.

Mesenchymal stromal cells (MSCs) are tissue progenitors with remarkable plasticity. Understanding their gene regulatory networks is key for tissue homeostasis, disease insights, and cell-based therapies.

More Related Videos

Epigenetic Regulation of Cardiac Differentiation of Embryonic Stem Cells and Tissues
13:03

Epigenetic Regulation of Cardiac Differentiation of Embryonic Stem Cells and Tissues

Published on: June 3, 2016

8.3K
Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal
08:01

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal

Published on: May 30, 2012

10.7K

Related Experiment Videos

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes
10:48

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes

Published on: April 12, 2015

10.3K
Epigenetic Regulation of Cardiac Differentiation of Embryonic Stem Cells and Tissues
13:03

Epigenetic Regulation of Cardiac Differentiation of Embryonic Stem Cells and Tissues

Published on: June 3, 2016

8.3K
Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal
08:01

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal

Published on: May 30, 2012

10.7K

Area of Science:

  • Cell biology
  • Developmental biology
  • Regenerative medicine

Background:

  • Stromal progenitors are crucial for tissue homeostasis and can differentiate into parenchymal cells.
  • In vitro culture reveals mesenchymal stromal cells (MSCs) possess significant lineage plasticity.
  • Tissue microenvironments differentially program these progenitor cells.

Purpose of the Study:

  • To explore the gene regulatory networks governing MSC lineage determination and differentiation.
  • To understand the role of signaling pathways and transcriptional regulators in MSC differentiation.
  • To leverage insights for understanding stromal cell contributions to disease and developing cell therapies.

Main Methods:

  • Utilizing loss-of-function studies (in vitro and in vivo).
  • Applying advanced omics and single-cell technologies.
  • Analyzing gene regulatory networks controlling cell differentiation.

    • Main Results:

    • Identified key signaling pathways and transcriptional regulators involved in MSC differentiation.
    • Uncovered sequential and coordinated mechanisms activating lineage-selective gene programs.
    • Gained system-wide insights into gene networks driving cell fate decisions.

    Conclusions:

    • MSC plasticity is regulated by complex gene networks.
    • Understanding these networks is vital for regenerative medicine and disease research.
    • Advances in omics technologies facilitate deeper insights into cell differentiation processes.