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Related Concept Videos

Deformation in a Circular Shaft01:10

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One of the distinctive characteristics of circular shafts is their ability to maintain their cross-sectional integrity under torsion. In other words, each cross-section continues to exist as a flat, unaltered entity, simply rotating like a solid, rigid slab. To understand the distribution of shearing stress within such a shaft, consider a cylindrical section inside this circular shaft. This section has a length of L and a radius of R, with one end fixed. The radius of the cylindrical section is...
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Circular Shaft - Stresses in Linear Range01:13

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Consider a scenario where a circular shaft is subject to torque that remains within the boundaries of Hooke's Law, avoiding any permanent deformation. So, the formula for shearing strain is revisited. This formula is multiplied by the modulus of rigidity, and then Hooke's Law for the shearing stress and strain is applied. As a result, the equation for shearing stress in a shaft can be derived.
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Related Experiment Video

Updated: Nov 9, 2025

Identification of Circular RNAs using RNA Sequencing
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Circular sisRNA identification and characterisation.

Amanda Yunn Ee Ng1, Jun Wei Pek1

  • 1Temasek Life Sciences Laboratory, 1 Research Link National University of Singapore, Singapore 117604, Singapore; Department of Biological Sciences, National University of Singapore, 14 Science Drive, Singapore 117543, Singapore.

Methods (San Diego, Calif.)
|April 10, 2021
PubMed
Summary
This summary is machine-generated.

Stable Intronic Sequence RNA (sisRNA) are newly discovered non-coding RNAs involved in gene regulation. This review details a workflow to enrich and detect these low-abundance sisRNAs using RNA sequencing, enabling further study.

Keywords:
Circular RNADrosophilaIntronic SequenceRNA-seqRNase RsisRNA

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Area of Science:

  • Molecular Biology
  • Genomics
  • Non-coding RNA Research

Background:

  • Stable Intronic Sequence RNA (sisRNA) are emerging non-coding RNAs with regulatory functions.
  • Identifying sisRNA via standard RNA sequencing is challenging due to their low abundance and masking by abundant RNA species.
  • sisRNA play roles in gene expression regulation through feedback loops.

Purpose of the Study:

  • To present a practical workflow for enriching and detecting circular sisRNA.
  • To enable reliable identification of low-abundance sisRNA using deep sequencing.
  • To provide methods for validating sisRNA circularity, stability, and function.

Main Methods:

  • Utilizing transcriptionally quiescent systems for enrichment.
  • Employing ribosomal RNA (rRNA)-depletion strategies.
  • Applying RNase R treatment to isolate stable RNA species.
  • Deep sequencing for comprehensive identification.

Main Results:

  • A workflow enabling reliable detection of circular sisRNA was established.
  • The methods facilitate the identification of previously masked sisRNA reads.
  • Experimental validation techniques for circularity and stability were presented.

Conclusions:

  • The described workflow significantly improves the detection of circular sisRNA.
  • This approach aids in understanding the regulatory roles of sisRNA in gene expression.
  • Further functional characterization of sisRNA is facilitated by these methods.