Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal01:22

Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal

2.4K
Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
2.4K
Allergic Reactions02:06

Allergic Reactions

29.7K
Overview
29.7K
Receptor Downregulation in MVBs01:15

Receptor Downregulation in MVBs

2.5K
Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that  lead to cell proliferation, migration, and differentiation. Overexpression of EGFR  stimulates cells to proliferate. Excessive  EGFR...
2.5K
Antiasthma Drugs: Mast Cell Stabilizers and Anti-IgE Drugs01:25

Antiasthma Drugs: Mast Cell Stabilizers and Anti-IgE Drugs

1.5K
Asthma is a chronic respiratory condition for which new therapeutic avenues, including anti-inflammatory drugs like mast cell stabilizers and anti-IgE treatments, continue to be developed.
Mast cell stabilizers, such as cromolyn (also known as sodium cromoglycate) and nedocromil (Tilade), are effective drugs in asthma management. These stabilizers hinder histamine release by skillfully obstructing the activation of mast cells and other cellular entities. Notably, they navigate this task without...
1.5K
Antibody Structure01:10

Antibody Structure

62.4K
Overview
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
62.4K
GPCRs Regulate Adenylyl Cylase Activity01:09

GPCRs Regulate Adenylyl Cylase Activity

6.3K
Some GPCRs transmit signals through adenylyl cyclase (AC), a transmembrane enzyme. AC helps synthesize second messenger cyclic adenosine monophosphate (cAMP). AC catalyzes cyclization reaction and converts ATP to cAMP by releasing a pyrophosphate. The pyrophosphate is further hydrolyzed to phosphate by the enzyme pyrophosphatase, which drives cAMP synthesis to completion. However, cAMP is rapidly degraded to 5′ AMP by the enzymes phosphodiesterase (PDE), preventing overstimulation of...
6.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Efficiency of sperm donation: An analysis of 440 qualified sperm donors in Chongqing Human Sperm Bank].

Zhonghua nan ke xue = National journal of andrology·2020
Same author

The complete mitochondrial genome of the <i>Papilio paris</i> (Lepidoptera: Papilionidae).

Mitochondrial DNA. Part B, Resources·2020
Same author

Age and gender dependence of liver diffusion parameters and the possibility that intravoxel incoherent motion modeling of the perfusion component is constrained by the diffusion component.

NMR in biomedicine·2020
Same author

Acute invariant NKT cell activation triggers an immune response that drives prominent changes in iron homeostasis.

Scientific reports·2020
Same author

Development of a robust crystallization platform for immune receptor TREM2 using a crystallization chaperone strategy.

Protein expression and purification·2020
Same author

Diosmetin reduces bone loss and osteoclastogenesis by regulating the expression of TRPV1 in osteoporosis rats.

Annals of translational medicine·2020

Related Experiment Video

Updated: Nov 9, 2025

Detection of True IgE-expressing Mouse B Lineage Cells
09:40

Detection of True IgE-expressing Mouse B Lineage Cells

Published on: December 1, 2014

11.2K

The high affinity IgE receptor: a signaling update.

Ulrich Blank1, Hua Huang2, Toshiaki Kawakami3

  • 1Université de Paris, Centre de Recherche sur l'Inflammation, INSERM UMR1149, CNRS ERL8252, Faculté de Médecine site Bichat, Paris, France; Laboratoire d'Excellence Inflamex, Paris, France.

Current Opinion in Immunology
|April 10, 2021
PubMed
Summary

This study details how the high-affinity IgE receptor (FcεRI) on mast cells triggers hypersensitivity and inflammation. It reveals distinct signaling pathways based on antigen properties, influencing gene expression and cell responses.

More Related Videos

Humanized Mediator Release Assay as a Read-Out for Allergen Potency
10:22

Humanized Mediator Release Assay as a Read-Out for Allergen Potency

Published on: June 29, 2021

3.7K
Development of an in vitro model system for studying the interaction of Equus caballus IgE with its high-affinity receptor Fc&#949;RI
07:31

Development of an in vitro model system for studying the interaction of Equus caballus IgE with its high-affinity receptor FcεRI

Published on: November 1, 2014

12.6K

Related Experiment Videos

Last Updated: Nov 9, 2025

Detection of True IgE-expressing Mouse B Lineage Cells
09:40

Detection of True IgE-expressing Mouse B Lineage Cells

Published on: December 1, 2014

11.2K
Humanized Mediator Release Assay as a Read-Out for Allergen Potency
10:22

Humanized Mediator Release Assay as a Read-Out for Allergen Potency

Published on: June 29, 2021

3.7K
Development of an in vitro model system for studying the interaction of Equus caballus IgE with its high-affinity receptor Fc&#949;RI
07:31

Development of an in vitro model system for studying the interaction of Equus caballus IgE with its high-affinity receptor FcεRI

Published on: November 1, 2014

12.6K

Area of Science:

  • Immunology
  • Cell Biology
  • Molecular Biology

Background:

  • Mast cells play a crucial role in allergic reactions and inflammation via the high-affinity IgE receptor (FcεRI).
  • FcεRI signaling initiates immediate hypersensitivity and cytokine-chemokine cascades.
  • Understanding mast cell degranulation and gene transcription is key to modulating allergic responses.

Purpose of the Study:

  • To update the understanding of proximal and distant signaling events initiated by FcεRI.
  • To investigate how antigen characteristics influence FcεRI signaling outcomes.
  • To identify novel effectors and regulatory elements involved in FcεRI-mediated mast cell activation and gene expression.

Main Methods:

  • Analysis of mast cell degranulation and associated molecular machinery.
  • Description of phosphorylation- and calcium-responsive signaling effectors.
  • Next-generation sequencing to identify gene enhancers responsive to FcεRI stimulation.
  • Investigation of calcium dependency and super enhancer identification in mouse and human mast cells.

Main Results:

  • Distinct intracellular signaling events and outcomes are dictated by antigen concentration, affinity, and valency.
  • A complex molecular machinery mediates FcεRI signaling to cytoskeletal changes, granule transport, and membrane fusion.
  • New phosphorylation- and calcium-responsive effectors have been identified.
  • FcεRI signaling promotes de novo gene transcription, with identified enhancers responding to antigenic stimulation.
  • Ca2+-dependency of gene enhancers in human mast cells and stimulation-responsive super enhancers in mouse mast cells were revealed.
  • The transcription factor GATA2 primes these enhancers for response.

Conclusions:

  • FcεRI signaling is a complex process involving distinct pathways modulated by antigen properties.
  • Mast cell activation involves intricate molecular machinery controlling degranulation and gene expression.
  • The identification of novel signaling effectors and regulatory elements provides new targets for therapeutic intervention in allergic diseases.