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Validation of a diphtheria toxoid multiplex bead assay for serosurveys
Heather M Scobie1, Nino Khetsuriani1, Androulla Efstratiou2
1Global Immunization Division, Centers for Disease Control and Prevention, Atlanta, GA, USA.
Diagnostic Microbiology and Infectious Disease
|April 10, 2021
Abstract:
We validated a multiplex bead assay for diphtheria toxoid IgG antibodies against the Vero cell toxin neutralization test using 1300 specimens (correlation = 0.88). At the ≥0.01 IU/mL cutoff for minimal seroprotection, sensitivity was 95% and specificity was 83%. Agreement for three categories (<0.01, 0.01-<0.1, ≥0.1 IU/mL) was 81% (kappa = 0.71).
