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Large scale model lipid membrane movement induced by a cation switch.

Laura H John1, Gail M Preston2, Mark S P Sansom3

  • 1Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK; ISIS Pulsed Neutron and Muon Source, Science and Technology Facilities Council, Rutherford Appleton Laboratory, Harwell Science and Innovation Campus, Didcot, Oxfordshire OX11 OQX, UK.

Journal of Colloid and Interface Science
|April 11, 2021
PubMed
Summary

Cation concentration changes reversibly alter biomembrane distance. This novel system, using a free-floating bilayer and self-assembled monolayer (SAM), precisely controls membrane-to-surface spacing for advanced biological studies.

Keywords:
Biological membranesBiomimeticBiosensorsCalciumCation bindingCation switchDistance tuningElectrostaticsModel membranesMolecular dynamicsNeutron reflectometrySelf-assembled monolayer

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Area of Science:

  • Biomembrane science
  • Surface science
  • Materials science

Background:

  • Understanding biomembrane behavior is crucial for cell biology and drug development.
  • Controlling membrane-surface interactions is challenging but essential for studying cellular processes.

Purpose of the Study:

  • To describe a novel biomembrane sample system enabling large-scale, reversible changes in membrane-to-surface distance.
  • To investigate the influence of cations (Ca2+ and Na+) on the behavior of free-floating bilayers adjacent to self-assembled monolayers (SAMs).

Main Methods:

  • Fabrication of a free-floating bilayer adjacent to a self-assembled monolayer (SAM).
  • Utilized neutron reflectivity and quartz crystal microbalance measurements to analyze membrane movements.
  • Performed molecular dynamics simulations to elucidate cation-induced mechanisms.

Main Results:

  • Millimolar cation concentration changes induced reversible alterations (≥ 200 Å) in membrane-to-surface distance.
  • Neutron reflectivity demonstrated precision manipulation of membrane-to-surface distance by varying Ca2+ and Na+ concentrations.
  • Simulations revealed Ca2+ bridges between SAM and bilayer, while Na+ binding was insufficient to overcome electrostatic repulsion.

Conclusions:

  • The developed biomembrane system offers precise control over membrane-to-surface distance, driven by cation concentration.
  • This easily producible system, validated by neutron reflectivity and quartz crystal microbalance, shows potential as a standard tool.
  • Applications include investigating membrane binding, endocytosis, and cell signaling pathways.