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Method for detecting hemodynamic alterations following a single gavage in rats
Taiki Fushimi1, Yasuyuki Fujii1, Hideaki Koshino2
1Functional Control Systems, Graduate School of Engineering and Science, Shibaura Institute of Technology, 307 Fukasaku, Minumaku, Saitama, 337-8570, Japan.
Experimental Animals
|April 12, 2021
Summary
A new gavage method in rodents minimizes cardiac reflexes, enabling accurate hemodynamic measurements. This technique allows for clear detection of circulatory changes after administering pungent or astringent compounds.
Area of Science:
- Physiology
- Pharmacology
- Surgical Techniques
Background:
- Gavage administration in rodents can trigger cardiac reflexes, confounding hemodynamic studies.
- Pungent or astringent compounds alter circulation via sympathetic activity, further complicating measurements.
Purpose of the Study:
- To develop a modified gavage method that minimizes hemodynamic alterations in rats.
- To validate the new method for assessing hemodynamic responses to oral administration of specific compounds.
Main Methods:
- A modified gastric tube (10 cm mark) and controlled instillation (37°C water, 1.0 ml/min) were used in urethane-anesthetized Wistar rats.
- Hemodynamic parameters including mean blood pressure (MBP), heart rate (HR), and cremasteric blood flow (BF) were measured.
- Comparisons were made between the new method and a conventional gavage technique.
Main Results:
- The optimized gavage method significantly reduced somatic afferent reflex-induced hemodynamic changes compared to the standard method.
- The new technique successfully allowed detection of transient increases in HR and MBP after capsaicin administration.
- Elevated cremasteric blood flow was observed following cinnamtannin A2 treatment, confirming the method's utility.
Conclusions:
- The developed gavage method effectively minimizes confounding hemodynamic alterations in rodent studies.
- This technique is suitable for evaluating the precise hemodynamic effects of orally administered substances like capsaicin and cinnamtannin A2.
