Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Preparation and analysis of tobacco glycosides, and the relationship between glycoside aglycones and pyrolysis products: a review.

Frontiers in molecular biosciences·2026
Same author

Baseline gut microbiome features associated with fecal calprotectin response to exclusive enteral nutrition in pediatric Crohn's disease.

Frontiers in pediatrics·2026
Same author

Lithium-Rich Antifluorite Li<sub>5</sub>Fe<i><sub>x</sub></i>Al<sub>1-<i>x</i></sub>O<sub>4</sub>: Phase-Controllable Syntheses, Local Structural Evolution, and Electrochemical Performance.

Inorganic chemistry·2026
Same author

MRI features of craniopharyngiomas in different age groups and pathological subtypes.

Frontiers in oncology·2026
Same author

Recent advances in decoding biosynthetic pathways and synthetic biology approaches for plant alkaloids.

Natural product reports·2026
Same author

Indirect reciprocity with environmental feedback.

Chaos (Woodbury, N.Y.)·2026

Related Experiment Video

Updated: Nov 9, 2025

Attaching Biological Probes to Silica Optical Biosensors Using Silane Coupling Agents
09:35

Attaching Biological Probes to Silica Optical Biosensors Using Silane Coupling Agents

Published on: May 1, 2012

13.2K

Boronic Acid Functionalized Nanosilica for Binding Guest Molecules.

Xiaoting Xue1, Haiyue Gong1, Hongwei Zheng1

  • 1Division of Pure and Applied Biochemistry, Department of Chemistry, Lund University, Box 124, 22100 Lund, Sweden.

ACS Applied Nano Materials
|April 12, 2021
PubMed
Summary

Functionalizing nanoscale pores in dendritic fibrous nanosilica (DFNS) is challenging. Using a polymer intermediate and click chemistry, researchers successfully immobilized boronic acid ligands, enhancing DFNS binding capacity for cis-diols.

More Related Videos

Preparation of Functional Silica Using a Bioinspired Method
08:04

Preparation of Functional Silica Using a Bioinspired Method

Published on: August 1, 2018

17.5K
Synthesis, Functionalization, and Characterization of Fusogenic Porous Silicon Nanoparticles for Oligonucleotide Delivery
08:53

Synthesis, Functionalization, and Characterization of Fusogenic Porous Silicon Nanoparticles for Oligonucleotide Delivery

Published on: April 16, 2019

7.9K

Related Experiment Videos

Last Updated: Nov 9, 2025

Attaching Biological Probes to Silica Optical Biosensors Using Silane Coupling Agents
09:35

Attaching Biological Probes to Silica Optical Biosensors Using Silane Coupling Agents

Published on: May 1, 2012

13.2K
Preparation of Functional Silica Using a Bioinspired Method
08:04

Preparation of Functional Silica Using a Bioinspired Method

Published on: August 1, 2018

17.5K
Synthesis, Functionalization, and Characterization of Fusogenic Porous Silicon Nanoparticles for Oligonucleotide Delivery
08:53

Synthesis, Functionalization, and Characterization of Fusogenic Porous Silicon Nanoparticles for Oligonucleotide Delivery

Published on: April 16, 2019

7.9K

Area of Science:

  • Materials Science
  • Nanotechnology
  • Chemical Engineering

Background:

  • Dendritic fibrous nanosilica (DFNS) possesses high surface area and nanochannels, making it suitable for catalysis, sensing, and bioseparation.
  • Functionalizing DFNS within its confined nanochannels presents significant challenges.
  • Developing methods for effective ligand immobilization on DFNS is crucial for advanced applications.

Purpose of the Study:

  • To investigate the conjugation of molecular ligands within the nanoscale pores of DFNS.
  • To explore the use of copper-catalyzed click reaction with an optional polymer intermediate for ligand functionalization.
  • To characterize the binding properties of functionalized DFNS using a model boronic acid ligand.

Main Methods:

  • Copper-catalyzed click reaction for ligand conjugation.
  • Synthesis of an in situ temperature-responsive polymer intermediate.
  • Characterization using electron microscopy, TGA, elemental analysis, FTIR, and N2 adsorption-desorption.
  • Binding studies with cis-diol molecules (Alizarin Red S, NAD) and glycoproteins.

Main Results:

  • Successful immobilization of boronic acid ligands onto DFNS via click chemistry.
  • Quantified ligand densities of 0.08 and 0.68 mmol/g for polymer-free and polymer-assisted DFNS, respectively.
  • Polymer-assisted DFNS demonstrated enhanced binding capacity for cis-diols compared to polymer-free DFNS.
  • Both functionalized DFNS types selectively bound small cis-diols over large glycoproteins due to size exclusion.

Conclusions:

  • Ligand conjugation within DFNS nanochannels is achievable using click chemistry, particularly with a polymer intermediate.
  • The polymer intermediate significantly enhances ligand loading and binding capacity.
  • Functionalized DFNS exhibits selective binding of small molecules, leveraging its unique nanochannel structure.
  • This approach offers a promising strategy for developing advanced DFNS-based materials for separation and sensing.