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Updated: Nov 9, 2025

In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions
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In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions

Published on: October 21, 2022

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Circular RNA circ_HECTD1 regulates cell injury after cerebral infarction by miR-27a-3p/FSTL1 axis

Zhenduo Zhang1, Jinbo He2, Baoliang Wang1

  • 1Department of Encephalopathy Third Ward, The First Affiliated Hospital of Henan University of CM, Zhengzhou, Henan, China.

Cell Cycle (Georgetown, Tex.)
|April 12, 2021
PubMed

Insights

Circular RNAs HECTD1 (circ_HECTD1) protects against cerebral infarction by regulating the microRNA-27a-3p/FSTL1 axis. This finding offers a potential therapeutic strategy for cerebral infarction, a condition with high mortality.

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Genetics

Background:

  • Cerebral infarction, a leading cause of mortality, involves neural cell injury.
  • Oxygen-glucose deprivation/reperfusion (OGD/R) models are crucial for studying cerebral ischemia.
  • Circular RNAs are increasingly recognized for their roles in cellular processes and disease.

Purpose of the Study:

  • To investigate the role and mechanism of circ_HECTD1 in OGD/R-induced neuronal injury.
  • To explore the potential of circ_HECTD1 as a therapeutic target for cerebral infarction.

Main Methods:

  • Real-time quantitative PCR (RT-qPCR) for gene expression analysis.
  • Cell viability, proliferation (EdU, MTT), and apoptosis assays (flow cytometry).
  • Western blot for protein expression and dual-luciferase reporter assay for molecular interactions.

Main Results:

  • Circ_HECTD1 and FSTL1 expression increased, while miR-27a-3p decreased in OGD/R-treated cells.
  • Knockdown of circ_HECTD1 enhanced cell proliferation and reduced apoptosis in OGD/R conditions.
  • Circ_HECTD1 acts as a molecular sponge for miR-27a-3p, regulating FSTL1 expression.

Conclusions:

  • Circ_HECTD1 deficiency mitigates OGD/R-induced neuronal damage by modulating the miR-27a-3p/FSTL1 axis.
  • The circ_HECTD1/miR-27a-3p/FSTL1 pathway represents a promising therapeutic target for cerebral infarction.

Keywords:
Circ_hectd1cerebral infarctionfstl1miR-27a-3poxygen-glucose deprivation

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