A combined approach based on matrix solid-phase dispersion extraction assisted by titanium dioxide nanoparticles and...

A simple and efficient low-cost matrix solid phase dispersion (MSPD) extraction assisted by TiO₂ nanoparticles and diatomaceous earth has been developed for the extraction of phenolic compounds from grape and grape pomace wastes. Experimental conditions for MSPD extraction were optimized by a factorial design and a surface response methodology. The simultaneous identification and quantification of eight main natural polyphenols (caffeic, p-coumaric, dihydroxybenzoic and gallic acid, rutin, resveratrol, quercetin and catechin) was possible by combining MSPD and capillary liquid chromatography coupled to a diode array detection and a mass simple quadrupole analyzer (cLC-DAD-MS). Good linearity and acceptable LOD (0.05-62 µg·g^-1) and LOQ (0.2-207 µg·g^-1) were obtained. The quantities of extracted polyphenols were within 2.4 and 333 µg·g^-1, with catechin and rutin the most abundant compounds in grape pomace and grape wastes, respectively. Furthermore, considering the prospective uses of the winery bioresidues, the extracts have been characterised in terms of bioactive properties (several antioxidant activities and bacterial inhibition against Staphylococcus aureus, Escherichia coli and Pseudomona aeruginosa) and parameters such as total polyphenol and total flavonoid content. The high antioxidant activity (IC₅₀ 5.0 ± 0.4 µg ·g^-1 against DPPH radical) and antibacterial activity (2.2 ± 0.3 mg·mL^-1) suggests that the methodology developed is efficient, rapid and promising for the extraction of phenolic compounds with potential application as bioactive ingredients in food and cosmetic industries.