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Antibodies labeled with 199Au: potential of 199Au for radioimmunotherapy
P Anderson1, A T Vaughan, N R Varley
1Department of Immunology, University of Birmingham Medical School, U.K.
International Journal of Radiation Applications and Instrumentation. Part B, Nuclear Medicine and Biology
|January 1, 1988
Abstract:
We have investigated the direct labeling of antibodies with gold isotopes as an alternative to iodine and chelated metals for antibody guided therapy. Both 199Au and 195Au complex anions in citrate-buffered solutions (pH 3.8-7.2) are rapidly bound to sheep polyclonal anti-CEA with high efficiency. After purification by gel filtration, the antibody retained 80% of the bound gold for upto 6 days at 4 degrees C and was reactive with its target antigen in a solid phase assay. Incubation with the gold binding substance D-penicillamine reduced labeling efficiency and stability.