Growth phase related variation in the radiation sensitivity of human colon adenocarcinoma cells

Changes in radiation sensitivity with length of time in culture are described for an early passage human colon adenocarcinoma cell line (WiDr). The cells were most radioresistant at the end of lag-phase, 2 days after subculture (Do = 1.7 Gy; n = 10). Radiation sensitivity then increased with time reaching a maximum during plateau-phase, between days 8 and 10 (Do = 1.0 Gy; n = 13). The oxygen enhancement ratio remained constant across the different growth phases of the culture. Cell volume decreased with time in culture as did the proportion of S- and G2M-phase cells. Flow cytometric analyses revealed an increase in the proportion of G1 type cells with a plateau between days 8 and 12 of around 75%. The cell age response measured from synchronized cells following 8 Gy showed that WiDr cells were most radiation resistant in mid S-phase with maximal sensitivity during G1. These cells did not show repair of potentially-lethal radiation damage but were efficient in the repair of sub-lethal damage. The ability to repair sub-lethal damage did not change with culture age.