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Quantification of Porcine Complement Activation Fragment C3a by a Neoepitope-Based Enzyme-Linked Immunosorbent Assay
Per H Nilsson1,2, Kristin Pettersen3, Martin Oppermann4
1Department of Immunology, University of Oslo and Oslo University Hospital Rikshospitalet, Oslo, Norway. per.nilsson@medisin.uio.no.
Abstract:
Enzyme-linked immunosorbent assay (ELISA) enables fast and simple quantification of analytes in the pico- to nanogram range in complex samples. Here, we describe an ELISA for the detection of porcine C3a as a marker for complement activation. Antibody specificity is critical for a robust assay. This assay is based on a pair of antibodies specific for the porcine C3a molecule and thus does not react with native C3.
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