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Complement System

Complement System

The complement system is a group of approximately 20 plasma proteins that strengthen the body's defenses against infections through opsonization, inflammation, and cell lysis. Opsonization involves coating pathogens with complement proteins, making them more recognizable and facilitating phagocyte engulfment. Certain complement proteins induce inflammation that attracts immune cells to the site of infection. Cell lysis involves the destruction of pathogens through the formation of a...

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Related Experiment Video

Updated: Nov 9, 2025

Methods for Quantitative Detection of Antibody-induced Complement Activation on Red Blood Cells

Methods for Quantitative Detection of Antibody-induced Complement Activation on Red Blood Cells

Published on: January 29, 2014

Complement C3 Deposition on Endothelial Cells Revealed by Flow Cytometry.

Idris Boudhabhay¹, Anne Grunenwald¹, Lubka T Roumenina²

¹Centre de Recherche des Cordeliers, INSERM, Sorbonne Université, USPC, Université Paris Descartes, Université Paris Diderot, Paris, France.

Methods in Molecular Biology (Clifton, N.J.)

|April 13, 2021

Summary

Measuring complement C3 deposition on endothelial cells via flow cytometry is a sensitive method to detect complement activation. This technique aids in understanding complement-mediated endothelial cell injury in conditions like atypical hemolytic uremic syndrome.

Keywords:

C3 Cell imaging Complement system Endothelial cells Flow cytometry

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Last Updated: Nov 9, 2025

Methods for Quantitative Detection of Antibody-induced Complement Activation on Red Blood Cells

Methods for Quantitative Detection of Antibody-induced Complement Activation on Red Blood Cells

Published on: January 29, 2014

Measuring Erythrocyte Complement Receptor 1 Using Flow Cytometry

Measuring Erythrocyte Complement Receptor 1 Using Flow Cytometry

Published on: May 19, 2020

In Vitro Microfluidic Disease Model to Study Whole Blood-Endothelial Interactions and Blood Clot Dynamics in Real-Time

In Vitro Microfluidic Disease Model to Study Whole Blood-Endothelial Interactions and Blood Clot Dynamics in Real-Time

Published on: May 24, 2020

Area of Science:

Immunology
Cell Biology

Background:

The complement system, a crucial part of innate immunity, involves three pathways converging on C3 cleavage.
C3b, an activated fragment of C3, covalently binds to surfaces, marking targets for immune attack.
Endothelial cells are significant targets of complement-mediated damage in diseases such as atypical hemolytic uremic syndrome.

Purpose of the Study:

To establish and validate flow cytometry for quantifying complement C3 deposition on endothelial cells.
To assess the utility of this method as a tool for studying complement activation in pathological conditions.

Main Methods:

Flow cytometry was employed to detect and quantify C3b deposition on endothelial cells.
Patient sera and various triggers of endothelial cell injury were used to induce complement activation.

Main Results:

Flow cytometry demonstrated high sensitivity in detecting complement C3 deposition on endothelial cells.
The method effectively measured complement activation induced by patient sera and identified complement-mediated endothelial cell injury.

Conclusions:

Flow cytometry for C3 deposition on endothelial cells is a sensitive and valuable tool for research and clinical applications.
This assay can assess complement activation in patient samples and evaluate the impact of complement mutations or cell injury triggers.