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Anti-C1-Inhibitor Autoantibody Detection by ELISA
Chiara Suffritti1, Sonia Caccia2, Silvia Berra2
1ASST-Fatebenefratelli-Sacco, Milan, Italy. chiarasuffritti@gmail.com.
Methods in Molecular Biology (Clifton, N.J.)
|April 13, 2021
Abstract:
Enzyme-linked immunosorbent assay (ELISA) is a quantitative analytical method used to measure the concentration of molecules in biological fluids through antigen-antibody reactions. Here we describe the measurement of anti-C1-inhibitor autoantibodies by an indirect ELISA. In this method patients' sera are incubated in a microplate coated with plasma derived C1-inhibitor.

