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Updated: Nov 9, 2025

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Stretching Short Sequences of DNA with Constant Force Axial Optical Tweezers
Published on: October 13, 2011
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Optical Tweezers to Investigate the Structure and Energetics of Single-Stranded DNA-Binding Protein-DNA Complexes
José A Morin1, Fernando Cerrón2, Francisco J Cao-García3,4
1Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.
Methods in Molecular Biology (Clifton, N.J.)
|April 13, 2021
Abstract:
Optical tweezers enable the isolation and mechanical manipulation of individual nucleoprotein complexes. Here, we describe how to use this technique to interrogate the mechanical properties of individual protein-DNA complexes and extract information about their overall structural organization.

