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Label-Free Quantitative Proteomics Workflow for Discovery-Driven Host-Pathogen Interactions

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Published on: October 20, 2020

New methods to decrypt emerging macropinosome functions during the host-pathogen crosstalk.

Yuen-Yan Chang^1,2, Jost Enninga¹, Virginie Stévenin^1,3,4

¹Institut Pasteur, Dynamics of Host-Pathogen Interactions Unit and CNRS UMR 3691, Paris, France.

Cellular Microbiology

|April 13, 2021

Summary

Macropinocytosis, a cellular process, is exploited by pathogens for entry and has other roles in disease. New methods reveal macropinosome dynamics and functions, aiding infection research.

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Area of Science:

Cell Biology
Pathogen-Host Interactions
Molecular Biology

Background:

Eukaryotic cells internalize extracellular material via macropinocytosis.
Intracellular pathogens hijack macropinocytosis for host cell entry.
Macropinosomes have diverse roles in pathology beyond fluid uptake.

Purpose of the Study:

To review novel methods for investigating macropinosomes.
To highlight insights gained into pathogen lifestyle and cellular functions.

Main Methods:

Imaging-based techniques for tracking macropinosome dynamics.
High-purity macropinosome extraction for proteomic analysis.

Main Results:

New methods provide deeper understanding of macropinosome identity and dynamics.
Insights into the intracellular lifestyle of Shigella and Salmonella.

Conclusions:

Advanced investigation methods are crucial for understanding macropinosomes.
Systematic characterization of macropinosome subversion in infections will advance cellular and molecular process knowledge.