Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Variation in the Respiration-to-Photosynthesis Ratio of Chinese Fir Saplings Is Associated with Foliar Non-Structural Carbohydrates.

Plants (Basel, Switzerland)·2026
Same author

Engineered Bacillus subtilis to deliver dsRNA via extracellular vesicles against the H9N2 avian influenza virus.

Trends in biotechnology·2026
Same author

Iterative Genome Engineering Platform Enables Efficient Sucrose Biosynthesis From CO<sub>2</sub> in Photosynthetic Synechococcus elongatus UTEX 2973.

Plant biotechnology journal·2026
Same author

Distinct associations of pioneer factor Ascl1-E12a with nucleosomes drive changes in cell fate.

Molecular cell·2026
Same author

Reducing P-O bond covalency in polyanionic groups to increase the intrinsic electronic conductivity in sodium positive electrodes.

Nature communications·2026
Same author

Single-cell transcriptomics and multimodal molecular profiling revealed the oncogenesis and tumor microenvironment of cardiac myxoma.

Science China. Life sciences·2026

Related Experiment Video

Updated: Nov 9, 2025

FLIM-FRET Measurements of Protein-Protein Interactions in Live Bacteria.
09:26

FLIM-FRET Measurements of Protein-Protein Interactions in Live Bacteria.

Published on: August 25, 2020

9.7K

A simple, switchable pili-labelling method by plasmid-based replacement of pilin.

Jingchao Zhang1, Shubin Li1, Tao Sun1,2

  • 1Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China.

Environmental Microbiology
|April 13, 2021
PubMed
Summary

We developed a simple pili-labeling method for studying bacterial Type IV pili (TFP). This technique enhances understanding of TFP roles in bacterial motility and pilin dynamics.

More Related Videos

Using Microtiter Dish Radiolabeling for Multiple In Vivo Measurements Of Escherichia coli pppGpp Followed by Thin Layer Chromatography
06:30

Using Microtiter Dish Radiolabeling for Multiple In Vivo Measurements Of Escherichia coli pppGpp Followed by Thin Layer Chromatography

Published on: June 4, 2019

6.3K
Digital PCR-based Competitive Index for High-throughput Analysis of Fitness in Salmonella
07:11

Digital PCR-based Competitive Index for High-throughput Analysis of Fitness in Salmonella

Published on: May 13, 2019

9.8K

Related Experiment Videos

Last Updated: Nov 9, 2025

FLIM-FRET Measurements of Protein-Protein Interactions in Live Bacteria.
09:26

FLIM-FRET Measurements of Protein-Protein Interactions in Live Bacteria.

Published on: August 25, 2020

9.7K
Using Microtiter Dish Radiolabeling for Multiple In Vivo Measurements Of Escherichia coli pppGpp Followed by Thin Layer Chromatography
06:30

Using Microtiter Dish Radiolabeling for Multiple In Vivo Measurements Of Escherichia coli pppGpp Followed by Thin Layer Chromatography

Published on: June 4, 2019

6.3K
Digital PCR-based Competitive Index for High-throughput Analysis of Fitness in Salmonella
07:11

Digital PCR-based Competitive Index for High-throughput Analysis of Fitness in Salmonella

Published on: May 13, 2019

9.8K

Area of Science:

  • Microbiology
  • Molecular Biology

Background:

  • Type IV pili (TFP) are crucial for bacterial functions like motility, surface sensing, and DNA uptake.
  • Current methods for TFP labeling often involve complex genetic modifications.

Purpose of the Study:

  • To present a straightforward, switchable pili-labeling method.
  • To characterize Pseudomonas aeruginosa pili morphology and twitching motility.
  • To investigate pilin pool replenishment dynamics.

Main Methods:

  • Developed a plasmid-based, inducible replacement strategy for PilA.
  • Avoided complex genetic modifications to the bacterial genome.
  • Utilized the method to analyze P. aeruginosa pili and motility.

Main Results:

  • Successfully labeled Type IV pili (TFP) using a simple, inducible plasmid system.
  • Detailed characterization of Pseudomonas aeruginosa pili morphology and twitching motility.
  • Demonstrated the method's utility in studying pilin pool replenishment dynamics.

Conclusions:

  • The developed pili-labeling method offers a simplified approach to study TFP functions.
  • This technique facilitates detailed analysis of bacterial motility and pilin dynamics.
  • Provides a valuable tool for microbiological research without complex genetic engineering.