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Updated: Nov 9, 2025
Cost-effective Method for Microbial Source Tracking Using Specific Human and Animal Viruses
Published on: December 3, 2011
Development and application of SYBR Green Ⅰ real-time quantitative reverse transcription PCR assay for detection of
Yin-He Xia1, Zi-Cong Shi1, Xin-Wei Wang1
1College of Veterinary Medicine, Henan Agricultural University, China.
Abstract:
Getah virus (GETV), a mosquito-borne virus belonging to the Alphavirus genus of family Togaviridae, has become increasingly problematic, which poses a huge threat to the safety of animals and public health. In order to detect GETV quickly and accurately, we have developed a SYBR Green I real-time quantitative reverse transcription PCR (RT-qPCR) assay for GETV with the detection limit of 66 copies/μL, excellent correlation coefficient (R2) of 0.9975, and amplification efficiency (E) of 98.90%, the target selected was the non-structural protein 3 of GETV. The sensitivity of it was higher than that of ordinary RT-PCR by 1000 folds, and the inter-assay and intra-assay CV values were all less than 0.99%. The newly developed RT-qPCR assay exhibited good sensitivity and reproducibility, which will provide technical support for the reliable and specific rapid diagnosis, and quantitative analysis of GETV infection.
