Related Experiment Video

Updated: Nov 9, 2025

Isolation of Cortical Microglia with Preserved Immunophenotype and Functionality From Murine Neonates
09:12

Isolation of Cortical Microglia with Preserved Immunophenotype and Functionality From Murine Neonates

Published on: January 30, 2014

16.4K

Activation of microglial G‑protein-coupled receptor 30 protects neurons against excitotoxicity through NF-κB/MAPK

Liu-Kun Yang1, Liang Lu1, Jiao Yue2

  • 1Department of Pharmacology, School of Pharmacy, Fourth Military Medical University, Xi'an, China.

Brain Research Bulletin
|April 13, 2021
PubMed

Insights

Activating microglial G-protein-coupled receptor 30 (GPR30) protects neurons from excitotoxicity by modulating microglial responses. This finding highlights microglial GPR30 as a potential therapeutic target for neurodegenerative diseases.

Area of Science:

  • Neuroscience
  • Immunology
  • Cell Biology

Background:

  • Neuroexcitotoxicity contributes to neuronal damage and neurodegenerative diseases.
  • Neuronal and astrocytic G-protein-coupled receptor 30 (GPR30) show neuroprotective effects.
  • The role of GPR30 in microglia during neuroexcitotoxicity is not well understood.

Purpose of the Study:

  • To investigate the function of microglial GPR30 in neuroprotection against glutamate-induced excitotoxicity.
  • To determine the effects of GPR30 activation on microglial behavior and inflammatory polarization.

Main Methods:

  • N9 microglial cells were treated with glutamate and a GPR30 agonist (G1).
  • Assays included MTT, Western blot, immunocytochemistry, phagocytosis, and wound healing.
  • Co-culture experiments with neurons and microglia were performed.

Main Results:

  • GPR30 activation by G1 inhibited glutamate-induced microglial proliferation and activation.
  • G1 treatment promoted M2 microglial polarization and reduced M1 polarization.
  • Microglial GPR30 activation protected neurons from excitotoxicity via NF-κB/MAPK signaling pathways.

Conclusions:

  • Microglial GPR30 plays a crucial role in neuroprotection against excitotoxicity.
  • Targeting microglial GPR30 may offer a therapeutic strategy for neurodegenerative conditions.
  • GPR30 modulates microglial polarization and inflammatory responses relevant to neuronal health.
Keywords:
GPR30GlutamateMicrogliaNeuroexcitotoxicity

More Related Videos

Primary Microglia Isolation from Mixed Glial Cell Cultures of Neonatal Rat Brain Tissue10:20

Primary Microglia Isolation from Mixed Glial Cell Cultures of Neonatal Rat Brain Tissue

Published on: August 15, 2012

40.1K
Modeling Neuronal Death and Degeneration in Mouse Primary Cerebellar Granule Neurons10:36

Modeling Neuronal Death and Degeneration in Mouse Primary Cerebellar Granule Neurons

Published on: November 6, 2017

8.3K

Related Concept Videos

NF-κB-dependent Signaling Pathway02:26

NF-κB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
8.3K
Ligand-Gated Ion Channel Receptor: Gating Mechanism01:30

Ligand-Gated Ion Channel Receptor: Gating Mechanism

Ligand-gated ion channels are transmembrane proteins that play a vital role in intercellular communication and functions of the nervous system. They allow the influx of ions across the membrane once the neurotransmitter binds, allowing the subsequent transmission of electrical excitation across the neurons. Other ligand-gated ion channels, like the γ-aminobutyric acid (GABA) receptor, permit anions like chloride into the cells on the binding of the GABA molecule. Their entry into the cell...
3.2K
Enzyme-linked Receptors01:00

Enzyme-linked Receptors

Enzyme-linked receptors are proteins that act as both receptor and enzyme, activating multiple intracellular signals. This is a large group of receptors that include the receptor tyrosine kinase (RTK) family. Many growth factors and hormones bind to and activate the RTKs.
Neurotrophin (NT) receptors are a family of RTKs, including trkA, trkB, and trkC (tropomyosin-related kinase) receptors. TrkA is specific for nerve growth factor (NGF), neurotrophin-6, and neurotrophin-7. TrkB binds...
82.2K