Construction of DCM-based NIR fluorescent probe for visualization detection of H₂S in solution and nanofibrous film

Hydrogen sulfide (H₂S) played crucial roles in biological processes and daily life, and the abnormal level of H₂S was associated with many physiological processes. In this paper, we designed and developed a dicyanomethylene-4H-chromene (DCM)-based near-infrared (NIR) fluorescent probe DCM-NO guided by theoretical calculation. The probe displayed excellent selectivity towards H₂S with a fast response time (3 min) and low detection limit (fluorescence 25.3 nM/absorption 6.61 nM) in Hela cells and real water samples. Furthermore, the probe-doped solid sensing materials (test strips and nanofibrous films) exhibited specific visualization of H₂S under ambient light or hand-held UV lamp, providing great potential for on-site and real-time application in environmental and biological systems.