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Lymphocyte expansion in bioreactors: upgrading adoptive cell therapy
Oscar Fabian Garcia-Aponte1, Christoph Herwig2, Bence Kozma1
1Research Area Biochemical Engineering, Institute of Chemical, Environmental and Bioscience Engineering, TU Wien, Gumpendorferstraße 1a, 1060, Vienna, Austria.
Journal of Biological Engineering
|April 14, 2021
Summary
Bioreactors are crucial for cell therapy but face challenges in controlling cell growth. This review analyzes automated lymphocyte expansion bioreactors, proposing design requirements and identifying advancement opportunities.
Area of Science:
- Cellular biology
- Biotechnology
- Immunotherapy
Background:
- Bioreactors are vital for producing high-quality cell therapy products.
- Current bioreactor applications for cell expansion face challenges in controlling cell growth and functionality.
- Optimizing bioreactor processes is key for therapy affordability and accessibility.
Purpose of the Study:
- To systematically review and compare current bioreactor technologies for automated lymphocyte expansion in adoptive cell therapy.
- To propose essential requirements for future bioreactor design.
- To identify trends, challenges, and advancements in bioreactor applicability for cell therapy.
Main Methods:
- Systematic analysis of published data on automated lymphocyte expansion.
- Comparative review of existing bioreactor technologies.
- Identification of key performance indicators and design considerations.
Main Results:
- Current bioreactor technologies present specific challenges and advancements in lymphocyte expansion.
- Process intensification offers opportunities for improved efficiency and scalability.
- A set of design requirements for bioreactors is proposed based on systematic analysis.
Conclusions:
- Targeted solutions are needed to address the unique needs of lymphocyte cultures (stimulation, supplementation, microenvironment).
- Knowledge-based tools can enhance process control and predictability in cell therapy manufacturing.
- Further development in bioreactor technology is essential to fully realize the potential of cell therapies.
