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[Correlation analysis between type 2 diabetes and core gut microbiota].

Y Xiao1, Y Niu1, M Mao1

  • 1School of Pharmacy, Minzu University of China, Key Laboratory of Ethnomedicine, Ministry of Education, Beijing 100081, China.

Nan Fang Yi Ke Da Xue Xue Bao = Journal of Southern Medical University
|April 14, 2021
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Summary
This summary is machine-generated.

Type 2 diabetes mellitus (T2D) is linked to significant changes in gut microbiota composition and function. These alterations indicate an ecological imbalance, highlighting potential therapeutic targets.

Keywords:
16S rRNAintestinal florametabolic pathwaystype 2 diabetes mellitus

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Area of Science:

  • Microbiome research
  • Metabolic disease research
  • Gut health and diabetes

Background:

  • Type 2 diabetes mellitus (T2D) is a global health concern.
  • The gut microbiome plays a crucial role in host metabolism and health.
  • Dysbiosis, or imbalance in gut flora, is increasingly associated with metabolic disorders like T2D.

Purpose of the Study:

  • To investigate the differences in intestinal flora species, abundance, and structure between T2D patients and healthy individuals.
  • To explore the correlation between alterations in gut microbiota and T2D.
  • To identify potential microbial biomarkers for T2D.

Main Methods:

  • High-throughput sequencing of the 16S rRNA gene V3+V4 regions from 133 fecal samples (55 T2D patients, 78 healthy controls).
  • Bioinformatic analyses using Usearch, QIIME, and R for diversity (Alpha and Beta) and differential abundance analysis (LefSe).
  • Functional prediction using PICRUST and KEGG pathway analysis.

Main Results:

  • Significant differences in gut flora diversity and composition between T2D patients and healthy individuals.
  • Dominance of Fimicutes and Bacteroidetes phyla in both groups, with significant differences in relative abundance at multiple taxonomic levels.
  • Identification of T2D-associated bacteria (e.g., Bifidobacterium, Lactobacillus) and upregulation of metabolic pathways (e.g., citric acid cycle, lipopolysaccharide biosynthesis) in T2D patients.

Conclusions:

  • The gut microbiome composition and abundance differ significantly between T2D and healthy individuals.
  • T2D is characterized by gut microbial ecological imbalance.
  • Identified microbial and metabolic pathway alterations provide insights into T2D pathogenesis and potential therapeutic strategies.