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Evaluation of an enzymatic method for determining creatinine in plasma
H Crocker1, M D Shephard, G H White
1Department of Biochemistry and Chemical Pathology, Flinders Medical Centre, Bedford Park, South Australia.
Journal of Clinical Pathology
|May 1, 1988
Summary
A new enzymatic method offers a more specific and efficient way to measure plasma creatinine, especially for neonates and diabetic patients. This optimized kit works well with centrifugal analyzers, improving accuracy over traditional methods.
Area of Science:
- Clinical Chemistry
- Biochemical Analysis
- Enzymatic Assays
Background:
- Plasma creatinine measurement is crucial for assessing kidney function.
- Traditional Jaffé-based methods for creatinine determination suffer from interferences and require larger sample volumes.
- Optimizing analytical methods is essential for accurate and efficient clinical diagnostics.
Purpose of the Study:
- To optimize an enzymatic kit method for plasma creatinine determination using a centrifugal analyzer.
- To compare the performance and practicality of the optimized enzymatic method against established Jaffé-based methods.
- To evaluate the specificity and potential interferences of the enzymatic creatinine assay.
Main Methods:
- Optimization of an enzymatic creatinine kit for use with a centrifugal analyzer.
- Comparative analysis of the enzymatic method versus endpoint and kinetic Jaffé-based methods.
- Assessment of interference from common substances like glucose, acetoacetate, cefoxitin, and bilirubin.
Main Results:
- The optimized enzymatic method demonstrated superior specificity compared to Jaffé-based methods.
- Advantages included a smaller sample size requirement and rapid sample throughput (200 samples/hour).
- Bilirubin caused a concentration-dependent negative interference, while glucose, acetoacetate, and cefoxitin did not interfere.
Conclusions:
- The optimized enzymatic creatinine assay offers significant advantages in specificity and efficiency.
- This method is particularly valuable for clinical applications involving neonates, diabetic ketotic patients, and individuals on cephalosporin therapy.
- The enzymatic method provides a reliable alternative to Jaffé-based assays, enhancing diagnostic accuracy in specific patient populations.